铜质凋亡是一种由增强的线粒体依赖性能量代谢引起的最新细胞死亡类型。本研究计划基于铜质凋亡相关基因（CRGs）建立结肠腺癌（COAD）患者的生存预后模型。我们使用癌症基因组图谱数据库对COAD的CRGs进行基因改变的调查，并在GSE41328数据集中进行验证。我们的结果显示，LIPT1、PDHA1、GLS和CDKN2A在COAD组织中的表达显著高于正常组织，而FDX1、DLD和MTF1在COAD组织中的表达显著低于正常组织（|(log2(fold change))| > 2，p < 0.05）。DLD（风险比（HR）：0.658；95%置信区间（CI）：0.445, 0.974；p = 0.037）和CDKN2A（HR：1.785；95% CI：1.200, 2.654；p = 0.004）的表达与整体生存相关，经过对数秩检验。CRG预后评分在1年、3年和5年时的曲线下面积分别为0.737、0.646和0.633。高风险因子的患者预后不良（HR = 1.514；95% CI：1.022, 2.243；p = 0.0386）。一个独立的验证数据集（GSE41328（N = 20））确认了以上结果。CRGs的特征可能作为COAD患者的预后预测因子，为抗癌治疗提供独特的见解。

Cuproptosis is the latest cell death type caused by enhanced mitochondrial-dependent energy metabolism. This study plans to establish a survival prognosis model for colon adenocarcinoma (COAD) patients based on cuproptosis-related genes (CRGs). We investigated the genetic alterations of CRGs in COAD based on The Cancer Genome Atlas database and validated in the GSE41328 dataset. Our results showed that LIPT1, PDHA1, GLS, and CDKN2A had significantly higher expression in COAD tissues than in normal tissues, while FDX1, DLD, and MTF1 had significantly lower expression in COAD tissues than in normal tissues (|(log2(fold change))| > 2, p < 0.05). DLD (hazard ratio (HR): 0.658; 95% confidence interval (CI): 0.445, 0.974; p = 0.037) and CDKN2A (HR: 1.785; 95% CI: 1.200, 2.654; p = 0.004) expressions were linked with overall survival throughout a log-rank test. CRG prognostic scores exhibited an area under the curve of 0.737, 0.646, and 0.633 at 1, 3, and 5 years. Patients with a high-risk factor suffered from poor prognosis (HR = 1.514; 95% CI: 1.022, 2.243; p = 0.0386). An independent validation dataset (GSE41328 (N = 20)) confirmed the above results. The CRGs' signature may be used as a prognostic predictor for COAD patients, providing unique insights into anticancer therapy.