非小细胞肺癌（NSCLC）是全球癌症相关死亡的主要原因之一。目前的肿瘤标志物通常在晚期诊断大部分病例。在这里，我们旨在鉴定NSCLC的潜在新生物标记物。从Gene Expression Omnibus数据库中分析了四个独立数据集。通过逆转录定量PCR测量了30对NSCLC组织中核苷酸还原酶调控亚单位 M2（RRM2）mRNA的相对表达水平。使用电化学发光免疫分析法测量了细胞角蛋白片段21-1（CYFRA21-1）、前胃泌素释放肽（ProGRP）、癌胚抗原（CEA）和神经元特异性烯醇酶（NSE）的血清水平，并通过酶联免疫吸附法评估了血清RRM2水平。与相邻组织相比，RRM2的mRNA表达水平在大多数NSCLC病变中明显增加。NSCLC患者的血清RRM2水平明显升高，与健康对照组相比，并且还与远处转移和组织学类型相关，但与肿瘤大小或淋巴结转移无关。接收者操作特征曲线分析显示，使用单独的RRM2相比于其他传统肿瘤标志物，在NSCLC的诊断比率上有更高的敏感性和特异性。RRM2是NSCLC的潜在血清诊断生物标志物。 版权声明：© 2023周等。本文是根据知识共享署名许可协议发布的开放获取文章的一部分，允许在任何媒介上无限制使用、分发和再产生，只要原始作者和来源得到了适当的认可。

Non-small cell lung cancer (NSCLC) is a major cause of cancer-related death worldwide. Most cases are diagnosed at an advanced stage using current tumor markers. Here, we aimed to identify potential novel potential biomarkers for NSCLC.Four independent datasets from the Gene Expression Omnibus database were analyzed. The relative expression of ribonucleotide reductase regulatory subunit M2 (RRM2) mRNA in 30 paired of NSCLC paired tissues was measured by reverse transcription quantitative PCR. Serum levels of cytokeratin fragment 21-1 (CYFRA21-1), pro-gastrin-releasing peptide (ProGRP), carcinoembryonic antigen (CEA), and neuron-specific enolase (NSE) were measured using electrochemiluminescence immunoassays, and serum RRM2 levels were evaluated by an enzyme-linked immunosorbent assay.The mRNA expression level of RRM2 was significantly increased in most NSCLC lesions compared to para-adjacent tissues. Serum RRM2 levels in NSCLC patients were significantly elevated compared to healthy controls and were also associated with distant metastasis and histological type, but not with tumor size or lymph node metastasis. Receiver operating characteristic curve analysis showed a higher diagnostic ratio for NSCLC using RRM2 alone compared to other traditional tumor markers.RRM2 is a potential sero-diagnostic biomarker for NSCLC.Copyright: © 2023 Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.