黄曲霉毒素B1（AFB1）对人类和动物健康构成重大风险，因为它污染了食物、饲料和谷物。这些危险的影响可以通过使用天然组分来减轻。本研究的目的是考察骆驼乳和水飞蓟素补充对大鼠中由黄曲霉毒素B1诱导的肝损伤的改善效果。通过测量白细胞和分类计数、血清生化指标和肝组织中肿瘤坏死因子（TNF-α）、抗氧化基因（NAD（P）H喹喔酮醌氧还蛋白还原酶1（NQO1））和碱基切除修复基因（APE1和OGG1）的基因表达，以及肝组织的病理学变化来评估这种改善。我们使用60只成熟的雄性雪鼠进行了本研究；这些大鼠被分成六组（每组十只）。对照组（无任何处理）通过灌胃给予生理盐水。骆驼乳组每日给予1毫升骆驼乳/公斤体重。水飞蓟素组每日给予1毫升水飞蓟素悬浮液溶液，剂量为20毫克水飞蓟素/公斤体重。黄曲霉素组每日给予含有1.4毫克黄曲霉素/千克饲料的黄曲霉素污染饮食，并给予生理盐水。骆驼乳+黄曲霉素组在同一时间内给予相同的骆驼乳口服剂量和黄曲霉素污染饮食。水飞蓟素+黄曲霉素组在同一时间内给予相同的水飞蓟素口服剂量和黄曲霉素污染饮食。所得数据表明黄曲霉毒素B1对白细胞计数、AST和ALT活性、血清蛋白、铁蛋白、甲胎蛋白、癌胚抗原、肝组织病理学和所研究基因的表达有害影响。然而，这些有害影响可以通过骆驼乳和水飞蓟素补充减轻。因此，我们可以得出结论：饮用骆驼乳和水飞蓟素可以减轻AFB1对大鼠血液学、AST和ALT活性、血清蛋白、铁蛋白、甲胎蛋白、癌胚抗原、肝组织病理学和基因表达的负面影响。©2023. Springer Nature Limited.

Aflatoxin B1 (AFB1) poses a major risk to both human and animal health because it contaminates food, feed, and grains. These dangerous effects can be mitigated using natural components. The purpose of this study was to examine the ameliorative effects of camel milk and silymarin supplementation upon aflatoxin B1 induced hepatic injury in rats. This improvement was assessed by measuring leukocytic and deferential counts, serum biochemical parameters, and gene expression of Tumor Necrosis Factor (TNF-α), antioxidant gene (NAD(P)H quinone oxidoreductase 1 (NQO1)), and base excision repair genes (APE1 and OGG1) in the liver tissue, in addition to liver histopathology. Sixty mature males Wister white rats were used to perform the present study; the rats were distributed in six groups (ten rats/group). The control group (without any treatment) received saline by gavage. The camel milk group received 1 ml of camel milk/kg body weight. The silymarin group received 1 ml of silymarin suspension solution at a dose of 20 mg of silymarin/kg of b.wt. The aflatoxin group received an aflatoxin-contaminated diet at a dose of 1.4 mg of aflatoxin /kg of diet and received saline. The camel milk + aflatoxin group received the same previous oral doses of camel milk and an aflatoxin-contaminated diet at the same time. The silymarin + aflatoxin group received the same previous doses of silymarin orally and an aflatoxin-contaminated diet at the same time. The obtained data indicated the deleterious effect of aflatoxin B1 on the leukocytic count, activity of AST and ALT, serum proteins, ferritin, alpha-fetoprotein, carcinoembryonic antigen, liver pathology, and the expression of the studied genes. However, these deleterious effects were mitigated by camel milk and silymarin supplementation. Thus, we could conclude that the ingestion of camel milk and silymarin mitigated the negative effects of AFB1 on the hematology, activity of AST and ALT, serum proteins, ferritin, alpha-fetoprotein, carcinoembryonic antigen, liver pathology, and gene expression in the rat model.© 2023. Springer Nature Limited.