探索中国中东部地区宫颈鳞状细胞癌（CSCC）组织中人乳头瘤病毒（HPV）的阳性率和基因型分布，为中国宫颈癌筛查和预防性HPV疫苗开发提供理论基础。从CSCC样本的石蜡包埋组织和宫颈癌筛查人群的脱落细胞中提取DNA。通过聚合酶链反应（PCR）和反向杂交基因芯片检测技术，检测了2,306例CSCC组织和10,245例宫颈癌筛查人群中的23个HPV基因型。分析了HPV感染的基因型分布。2,306例CSCC患者中的HPV总感染率为92.71%。单型HPV感染和多型HPV感染的频率分别为86.48%和13.51%。中国CSCC组织中最常见的HPV基因型是HPV-16、HPV-18、HPV-31、HPV-33、HPV-45、HPV-52、HPV-58和HPV-59。这些八个高风险HPV (HR-HPV) 基因型在HPV阳性的CSCC中的总阳性率高达96.91%。其中，与九价HPV疫苗相关的七个高风险HPV基因型在HPV阳性的CSCC中的阳性率为95.09%。与接受宫颈癌筛查的35-64岁女性相比，高风险HPV和低风险HPV的总感染率分别为13.16%和1.32%。宫颈癌筛查妇女中高频HR-HPV基因型是HPV-52、HPV-58、HPV-16、HPV-53、HPV-68、HPV-39、HPV-51和HPV-56，阳性率分别为2.25%、1.60%、1.31%、1.22%、0.93%、0.92%、0.78%和0.74%。在中国进行宫颈癌筛查的妇女中，检测8个高频HR-HPV基因型可以降低技术难度和试剂成本，同时提高宫颈癌筛查的效率和效果。HPV基因分型有助于妇科医生评估HR-HPV阳性宫颈上皮内瘤变的风险，并指导他们采取适当的干预措施。此外，HPV基因分型对医生跟踪HR-HPV阳性妇女和评估HPV疫苗的保护效果也是有帮助的。版权所有© 2023年韩、黄、叶、邹、兰、陈、姜、王、夏、罗、李、耿、王和黄。

To explore the positivity rate and genotype distribution of human papillomavirus (HPV) in cervical squamous cell carcinoma (CSCC) tissues in central and eastern China and to provide theoretical basis for cervical cancer screening and prophylactic HPV vaccine development in China.DNA was extracted from paraffin-embedded tissues of CSCC samples and exfoliated cervical cells of cervical cancer screening populations. 23 HPV genotypes were detected by combining polymerase chain reaction (PCR) and reverse dot hybridized gene chip detection technology in 2,306 CSCC tissues and 10,245 cervical cancer screening populations. The genotype distribution of HPV infection was analyzed.The overall infection rate of HPVs in 2,306 CSCC patients was 92.71%. The frequency of single-type HPV infection and multiple-type HPV infection were 86.48% and 13.51%, respectively. The most common HPV genotypes detected in Chinese CSCC tissues were HPV-16, HPV-18, HPV-31, HPV-33, HPV-45, HPV-52, HPV-58, and HPV-59. The overall positivity rate of these eight high-risk HPV (HR-HPV) genotypes in HPV-positive CSCC was as high as 96.91%. Of which the positivity rate of seven HR-HPV genotypes related to nine-valent HPV vaccines in HPV-positive CSCC was 95.09%. Meanwhile, the overall infection rates of HR-HPV and low-risk HPV (LR-HPV) in female aged 35-64 years who underwent cervical cancer screening were 13.16% and 1.32%, respectively. The high-frequency HR-HPV genotypes in cervical cancer screening women were HPV-52, HPV-58, HPV-16, HPV-53, HPV-68, HPV-39, HPV-51, and HPV-56, with positivity rates of 2.25%, 1.60%, 1.31%, 1.22%, 0.93%, 0.92%, 0.78%, and 0.74%, respectively.Among women screened for cervical cancer in China, detecting the 8 high-frequency HR-HPV genotypes can reduce technical difficulty and reagent costs, while also improving the efficiency and effectiveness of cervical cancer screening. HPV genotyping assists gynecologists in assessing the risk of HR-HPV-positive cervical intraepithelial neoplasia and guiding them in implementing appropriate interventions. Furthermore, HPV genotyping is helpful for doctors to follow up HR-HPV-positive women and to evaluate the protective effect of HPV vaccine.Copyright © 2023 Han, Huang, Ye, Zou, Lan, Chen, Jiang, Wang, Xia, Luo, Li, Geng, Wang and Huang.