胰腺导管腺癌（PAAD）是胃肠道的一种致命恶性肿瘤。环状RNA是一种内源性非编码RNA，被认为是肿瘤发生和发展中的新型调控分子。本文旨在调查circPGAM1在PAAD中的作用。将PAAD细胞株HPAC转染OE-circPGAM1以过表达circPGAM1并用AZD5363处理以抑制AKT/mTOR通路。同时，将另一个PAAD细胞株BxPC-3转染sh-circPGAM1以沉默circPGAM1。使用GEPIA数据库确定PAAD中circPGAM1的表达及其与总体生存和无病生存的关联。利用逆转录定量PCR确定细胞系中circPGAM1表达水平。进行细胞计数试剂盒-8、划痕愈合和transwell实验以确定细胞迁移和侵袭。使用西方印迹确定磷酸化AKT和mTOR的蛋白表达水平。在PAAD中过表达circPGAM1并与不良预后相关。沉默circPGAM1可抑制BxPC-3细胞的迁移和侵袭，而过表达circPGAM1则显示相反的效果。总的来说，circPGAM1通过AKT/mTOR轴促进了PAAD细胞的迁移和侵袭。© 2023. 作者与Springer Science+Business Media, LLC独家许可，属于Springer Nature的一部分。

Pancreatic adenocarcinoma (PAAD) is a lethal malignancy of the gastrointestinal tract. Circular RNA, an endogenous noncoding RNA, is considered a new regulatory molecule in tumorigenesis and development. Here, we aimed to investigate the role of circPGAM1 in PAAD. The PAAD cell line HPAC was transfected with OE-circPGAM1 to overexpress circPGAM1 and treated with AZD5363 to inhibit the AKT/mTOR pathway. Simultaneously, another PAAD cell line BxPC-3 was transfected with sh-circPGAM1 to silence circPGAM1. The GEPIA database was used to determine the expression of circPGAM1 in PAAD and its association with overall and disease-free survival. CircPGAM1 expression levels were determined in cell lines using reverse transcription-quantitative PCR. The cell counting kit-8, wound healing, and transwell assays were performed to determine cell migration and invasion. The protein expression levels of phosphorylated AKT and mTOR were determined using western blotting. CircPGAM1 was overexpressed in PAAD and related to poor prognosis. Silencing circPGAM1 inhibited migration and invasion of BxPC-3 cells, and overexpression of circPGAM1 showed the opposite effects. Overall, circPGAM1 promoted the migration and invasion of PAAD cells through the AKT/mTOR axis.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.