确定长链非编码RNA "MALAT1" 在口腔鳞状细胞癌（OSCC）患者唾液中的诊断准确性，并评估MALAT1靶向的microRNA-124在唾液中的表达。收集了四十个病例连续分型，并分为两组。A组包括20例OSCC患者，B组包括20名健康受试者。应用定量实时聚合酶链反应评估两组研究对象的唾液中MALAT1和microRNA-124的表达，并与OSCC病例的组织病理检查进行相关性分析。OSCC病例的MALAT1表达显著高于健康对照组，并且OSCC病例的miRNA-124表达显著低于对照组。唾液中的MALAT1和miRNA-124呈显著负相关关系。此外，与无转移病例相比，在有转移的病例中唾液中的MALAT1表达存在显著差异，并且在淋巴结受累的患者中与未受累的患者相比也存在显著差异。在2.24的截断值下，唾液中的MALAT1在鉴别OSCC和健康受试者方面表现出95%的敏感性和90%的特异性。唾液中的MALAT1作为miRNA-124的“海绵”可能成为一种潜在的OSCC唾液生物标志物。© 2023 Wiley Periodicals LLC.

To determine the diagnostic accuracy of the long non-coding RNA "MALAT1" measured in the saliva of patients with oral squamous cell carcinoma (OSCC) and assess the salivary expression of microRNA-124, which MALAT1 targets.Forty subjects were collected in a consecutive pattern and allocated into two groups. Group A included 20 patients with OSCC, while Group B included 20 healthy subjects. Salivary expression of MALAT1 and microRNA (miRNA)-124 was evaluated in the two study groups using quantitative real-time polymerase chain reaction and correlated with histopathological examination of OSCC subjects.OSCC yielded a statistically significant higher expression of MALAT1 than healthy controls and a lower expression of miRNA-124 in OSCC than controls. There is a statistically significant inverse relationship between salivary MALAT1 and miRNA-124. Moreover, there is a statistically significant difference in the MALAT1 expression in saliva samples from metastatic cases compared with non-metastatic cases, as well as in patients with lymph node involvement compared with those without involvement. At a cut-off value of 2.24, salivary MALAT1 exhibited 95% sensitivity and 90% specificity in differentiating OSCC from healthy subjects.Salivary MALAT1 acts as a sponge for miRNA-124 and could be a potential salivary biomarker for OSCC.© 2023 Wiley Periodicals LLC.