酪氨酸酶（TYR）在黑色素的生物合成过程中发挥着重要作用，其活性水平对白癜风、黑色素瘤和食物营养价值具有关键意义。敏感测定TYR活性对于基础研究和临床调查都具有重要意义。在本研究中，我们成功地通过一锅水热法合成了硅掺杂的碳量子点（Si-CQDs），以反式庚二酸为碳源，N-[3-(三甲氧基硅烷基)丙基]乙二胺为掺杂剂，展现出卓越的荧光量子效率（QY）和光稳定性。相应地，我们利用Si-CQDs作为探针构建了一种敏感、快速和便于操作的TYR荧光检测方法。该方法依赖TYR对异丙肾上腺素（ISO）的氧化作用，其中Si-CQDs被用作高效的探针。测试机制是Si-CQDs和ISO、TYR的氧化系统之间观察到的内部滤波效应（IFE）。在优化条件下，荧光策略对TYR表现出0.05-2.0 U/mL的检测范围，检测限（LOD）为0.041 U/mL。此外，我们成功地在人体血清中准确测定了TYR水平，展示了该方法在各种实际情景中的潜在应用前景。

Tyrosinase (TYR) plays a pivotal role in the biosynthesis of melanin, and its activity level holds critical implications for vitiligo, melanoma cancer, and food nutritional value. The sensitive determination of TYR activity is of great significance for both fundamental research and clinical investigations. In this work, we successfully synthesized silicon-doped carbon quantum dots (Si-CQDs) through a one-pot hydrothermal method with trans-aconitic acid as carbon source and N-[3-(Trimethoxysilyl)propyl]ethylenediamine as the dopant, exhibiting remarkable fluorescence quantum yield (QY) and photostability. Correspondingly, Si-CQDs were used as a probe to construct a sensitive, rapid, and user-friendly fluorescence method for TYR detection. The method relied on the oxidation of isoprenaline (ISO) by TYR, where Si-CQDs were employed as a highly efficient probe. The testing mechanism was the internal filtering effect (IFE) observed between Si-CQDs and the oxidative system of ISO and TYR. Under the optimized conditions, the fluorescence strategy exhibited a detection range of 0.05-2.0 U/mL for TYR with a limit of detection (LOD) of 0.041 U/mL. Furthermore, we successful demonstrated the accurate determination of TYR levels in human serum, showcasing the promising potential of this method in various practical scenarios.