食管鳞状细胞癌(ESCC)是最常见和最致命的肿瘤之一，然而，其潜在的分子机制尚未完全理解，需要新的治疗靶点。在这里，我们发现转录因子basonuclin 1 (BNC1)显著上调，并与ESCC的分化和转移密切相关。此外，BNC1、LINC01305和G蛋白信号通路抑制剂1(GPS1)在ESCC中具有显著的致癌作用。此外，体内实验显示，BNC1的沉默确实显著抑制了ESCC的增殖和转移。我们还揭示了LINC01305如何通过招募BNC1到GPS1的启动子，进而通过GPS1介导JNK信号通路促进ESCC的增殖和转移的分子机制。综上所述，我们发现了LINC01305/BNC1上调GPS1表达促进ESCC发展的新的分子机制，为ESCC提供了新的治疗靶点。© 2023 The Authors. 由澳大利亚约翰·怀利父子有限公司代表日本癌症协会发表的《癌症科学》。

EsophageaL squamous cell carcinoma (ESCC) is one of the most common and lethal tumors, however, its underlying molecular mechanisms are not completely understood and new therapeutic targets are needed. Here, we found that the transcription factor basonuclin 1 (BNC1) was significantly upregulated and closely related to the differentiation and metastasis of ESCC. Furthermore, BNC1, LINC01305, and G-protein pathway suppressor 1 (GPS1) had significant oncogenic roles in ESCC. In addition, in vivo experiments showed that knockdown of BNC1 indeed significantly inhibited the proliferation and metastasis of ESCC. We also revealed the molecular mechanism by which LINC01305 recruits BNC1 to the promoter of GPS1, and then GPS1 could mediate the JNK signaling pathway to promote the proliferation and metastases of ESCC. Taken together, we discovered the novel molecular mechanism by which LINC01305/BNC1 upregulates GPS1 expression to promote the development of ESCC, providing a new therapeutic target for ESCC.© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.