本研究调查了两种野生西西里岛特有植物Seseli bocconei Guss.和Seseli tortuosum subsp. maritimum Guss.不同部位（花、叶、茎和根）所提取的两种精油（EOs）的化学成分。结果显示，S. bocconei的精油主要成分包括单萜烃（花）、倍半萜烃（叶）和前两个类别的混合物（茎）。而S. tortuosum subsp. maritimum的精油无论是在花、茎还是根中，主要成分都是单萜烃，并含有一定比例的非萜类化合物。此外，我们评估了这些精油对结肠癌细胞HCT116的抗肿瘤效果。细胞活性实验显示，两种植物茎部的精油在低浓度下表现出强烈的细胞毒性效果，而其他植物器官的精油效果不显著。事实上，S. tortuosum subsp. maritimum的茎部精油在浓度为125μg/mL时细胞存活率降低了82%，而S. bocconei的茎部精油在浓度为250μg/mL时，97%的细胞死亡。对这些精油的主要植物成分（S-(-)-柠檬烯、R-(+)-柠檬烯、藿香烯、(1S)-(-)-α-蒎烯、(1R)-(+)-α-蒎烯、(-)-β-蒎烯和石菖蒲烯D）对结肠癌细胞的影响分析发现，石菖蒲烯D是一种具有抗癌性质的新型有前景的分子，值得在未来的研究中探索。版权所有©2023 Elsevier B.V. 发表。

In this study, the chemical compositions of two essential oils (EOs) obtained from different parts (flowers, leaves, stems, and roots) of Seseli bocconei Guss. and of Seseli tortuosum subsp. maritimum Guss., wild endemic species of Sicily, were investigated. The main classes of metabolites for the essential oils of S. bocconei were, respectively, monoterpenes hydrocarbons for flowers, sesquiterpenes hydrocarbons for leaves, and a breakdown between the two previously mentioned classes for stems. In the case of S. tortuosum subsp. maritimum, on the other hand, the main metabolite class for all the vegetative parts analyzed (flowers, stems, and roots) was monoterpene hydrocarbons, with a slight percentage in other non-terpenoid compounds. Furthermore, the EOs' antitumor effects against HCT116, human colon cancer cells were evaluated. Cell viability assays evidenced that stems' EOs of both plants exhibit strong cytotoxic effects at low concentrations, while the EOs from other vegetative parts do not show a relevant effect. In fact, EO of stems of S. tortuosum subsp. maritimum reduced the cell viability of 82% at the concentration of 125 μg/mL, while at the concentration of 250 μg/mL of stems EO of S. bocconei the 97% of cells resulted dead. The analysis of the effects exerted by the main phytocostituents (S-(-)-limonene, R-(+)-limonene, sabinene, (1S)-(-)-α-pinene, (1R)-(+)-α-pinene, and (-)-β-pinene, and germacrene D) of these EOs on colon cancer cells revealed germacrene D as a new promising molecule with anticancer properties that deserve to be explored in future directions.Copyright © 2023. Published by Elsevier B.V.