慢性溃疡性结肠炎（UC）患者患上结肠直肠癌（CRC）的几率较高。寄生虫感染对UC的影响被低估了。本研究的目的是研究肠道原虫感染对UC所产生的异型增生变化的影响。研究包括152例成人，其UC的组织学被证实，和152个健康对照组。粪便样本被检查是否存在寄生虫和粪便钙卤素蛋白（FC）。酶联免疫吸附测定法测定了血清抗p53抗体（p53Abs）和金属硫蛋白（MTs）。先进氧化蛋白产物（AOPPs）和还原型谷胱甘肽（GSH）水平在所有被试对象中通过分光光度法测定。血清C反应蛋白（CRP）和IL-6的测量也进行了。此外，对肠道组织进行了组织病理学和免疫组化研究。我们的结果显示，与无寄生虫的患者相比，溃疡性结肠炎患者合并寄生虫感染的FC、CRP、IL-6、AOPPs、MTs和p53Abs显著增加。相反，与其他组相比，相同组中的GSH水平显著降低。对肠道组织的组织病理学和免疫组织化学评估表明，与无寄生虫感染的患者相比，合并寄生虫感染的患者存在严重炎症和PD-L1的高表达。我们的研究表明，UC患者中肠道原虫的频率较高，其与炎症和异型增生指标水平升高有关。这表明这些寄生虫可能参与了慢性UC的病因和相关致癌过程。这是关于寄生虫感染和UC患者异型变化之间联系的首个报告。© 2023. The Author(s).

The chance of getting colorectal cancer (CRC) is higher in people with chronic ulcerative colitis (UC). The impact of parasitic infections on UC is underappreciated. The purpose of this study was to look into the effect of intestinal protozoal infections on the dysplastic changes generated by UC. The research included 152 adult patients with histologically confirmed UC and 152 healthy controls. Fecal samples were examined for the presence of parasites and fecal calprotectin (FC). The enzyme-linked immunosorbent assay measured serum anti-p53 antibodies (p53Abs) and metallothioneins (MTs). The advanced oxidation protein products (AOPPs) and reduced glutathione (GSH) levels were measured by a spectrophotometric method in all subjects. Serum C-reactive protein (CRP) and IL-6 were also measured. In addition, histopathological and immunohistochemical investigations of intestinal tissue were done. Our results exhibited significant increases in FC and CRP, IL-6, AOPPs, MTs, and p53Abs in ulcerative colitis patients with parasitic infections compared to those without parasites. In contrast, GSH levels showed a significant decrease in the same group compared with other groups. Histopathological and immunohistochemical assessments of intestinal tissue signified severe inflammation and strong expression of PD-L1 in patients with parasitic infections compared to others without parasitic infections. Our research indicated a greater frequency of intestinal protozoa in UC patients with elevated inflammatory and dysplastic biomarker levels. This suggests that these parasites may be involved in the etiology of chronic UC and the associated carcinogenetic process. This is the first report of a link between parasitic infections and dysplastic alterations in UC patients.© 2023. The Author(s).