白藜芦醇是一种有前途的肿瘤治疗药物，但其抗肿瘤机制尚未明确。本研究旨在探讨白藜芦醇对外泌体分泌的影响以及白藜芦醇诱导的外泌体在肝细胞癌发展中的作用。通过纳米颗粒追踪分析和高通量测序法分别确定了白藜芦醇诱导的外泌体的数量和内容。利用免疫荧光和免疫印迹分析了Rab27a的表达。通过刺激白藜芦醇和外泌体，观察了细胞增殖、迁移和上皮间质转变。通过免疫荧光和免疫印迹监测了白藜芦醇诱导的外泌体和lncRNA SNHG29敲除后自噬和wnt/β-连环蛋白信号通路的活性。研究发现，白藜芦醇可能通过下调Rab27a的表达抑制外泌体的分泌，从而抑制Huh7细胞的增殖、迁移和上皮间质转变。此外，白藜芦醇诱导的外泌体还可以通过抑制β-连环蛋白的核转位和自噬的活化来抑制Huh7细胞的恶性表型，可能通过lncRNA SNHG29介导。白藜芦醇通过调节外泌体的分泌和内容抑制肝细胞癌的进展。© Bentham Science Publishers；如有任何问题，请发送电子邮件至epub@benthamscience.net。

Resveratrol is a promising drug for tumor therapy, but its anti-tumor mechanism remains unclarified. The present study aimed to explore the effect of resveratrol on the secretion of exosomes and the role of resveratrol-induced exosomes in the progression of hepatocellular carcinoma.The number and contents of exosomes induced by resveratrol were determined by nanoparticle tracking analysis and high-throughput sequencing in Huh7 cells, respectively. Expression of Rab27a was assessed by western blotting and immunofluorescence. Cell proliferation, migration and epithelial-mesenchymal transition were examined with the stimuli of resveratrol and exosomes, the activity of autophagy and wnt/β-catenin signaling induced by resveratrol-induced exosomes and knockdown of lncRNA SNHG29 were monitored by western blotting and immunofluorescence.It was found that resveratrol might inhibit the exosome secretion by down-regulating the expression of Rab27a, thereby suppressing the proliferation, migration and epithelial-mesenchymal transition of Huh7 cells. Moreover, resveratrol-induced exosomes could also inhibit the malignant phenotype of Huh7 cells via inhibiting the nuclear translocation of β-catenin and the activation of autophagy, which lncRNA SNHG29 might mediate.Resveratrol inhibits hepatocellular carcinoma progression by regulating exosome secretion and contents.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.