人类T淋巴病毒-1（HTLV-1）感染患者IgG介导对健康外周T（CD4+，CD8+和γδ）和B细胞产生的IL-4，IL-10，IL-17和IFN-γ的差异调控。
Differential modulation of IL-4, IL-10, IL-17, and IFN-γ production mediated by IgG from Human T-lymphotropic virus-1 (HTLV-1) infected patients on healthy peripheral T (CD4+, CD8+, and γδ) and B cells.
发表日期：2023
作者：
Nicolle Rakanidis Machado, Beatriz Oliveira Fagundes, Lorena Abreu Fernandes, Augusto César Penalva de Oliveira, Youko Nukui, Jorge Casseb, Fernando Roberto Machado Cunha, Luiz Henrique da Silva Nali, Sabri Saeed Sanabani, Jefferson Russo Victor
来源：
CYTOKINE & GROWTH FACTOR REVIEWS
摘要：
人类T淋巴病毒1型（HTLV-1）感染者可作为无症状携带者（ACs）存在，也可能发展成慢性神经系统疾病HTLV-1相关脊髓病/热带痉挛性截瘫（HAM/TSP）或成人T细胞白血病/淋巴瘤（ATLL），需要进一步阐明这些病理过程中所涉及的免疫机制。最近，研究表明，从不同免疫状态的供体群体中获得的诱导或自然生成的IgG克隆群体可以调节人类T细胞和B细胞的功能。因此，本研究旨在评估HTLV-1感染的ACs、HAM/TSP和ATLL患者获得的IgG是否会不同程度地调节人类T细胞和B细胞的细胞因子产生。为达到此目的，我们将外周血单个核细胞（PBMCs）与来自ACs、HAM/TSP或ATLL供体的纯化IgG一起培养，并通过流式细胞术评估细胞因子的频率和细胞内产生情况。我们的研究结果表明，来自HAM/TSP患者的IgG可增加IL-17产生的CD4+T细胞数量，减少IL-4产生的CD4+T细胞数量，增加IFN-γ产生的CD8+T细胞数量，减少IL-4产生的CD8+T细胞数量。来自ATLL的IgG能够像来自HAM/TSP一样减少IL-4产生的CD4+T细胞数量，并能减少IFN-γ产生的γδT细胞的频率，但不影响IL-17和IL-4产生的γδT细胞的频率，并且能够减少IL-10产生的B细胞的频率。最后，来自HAM/TSP和ATLL患者的IgG都能够减少IFN-γ产生的B细胞的频率。总之，这些结果表明，这些制剂在作用上有部分重叠，能够对目标群体产生不同的影响。
版权所有 © 2023 Machado, Fagundes, Fernandes, Oliveira, Nukui, Casseb, Cunha, Nali, Sanabani and Victor.
Human T-lymphotropic virus 1 (HTLV-1) infected individuals remain as asymptomatic carriers (ACs) or can develop the chronic neurological disorder HTLV-1-associated myelopathy/Tropical Spastic Paraparesis (HAM/TSP) or the adult T-cell leukemia/lymphoma (ATLL), and the immunological mechanisms involved in this pathologies need to be elucidated. Recently, it has been demonstrated that induced or naturally developed IgG repertoires obtained from different groups of donors, grouped by immune status, can modulate human T and B cell functions. Here we aimed to evaluate if the IgG obtained from HTLV-1-infected ACs, HAM/TSP, and ATLL patients can differentially modulate the production of cytokines by human T and B cells. With this purpose, we cultured PBMCs with IgG purified from ACs, HAM/TSP, or ATLL donors and evaluated the frequency and intracellular cytokine production by flow cytometry. Our results indicate that IgG from HAM/TSP patients could induce an augment of IL-17-producing CD4+ T cells, reduce the frequency of IL-4-producing CD4+ T cells, increase IFN-γ-producing CD8+ T cells, and reduce IL-4-producing CD8+ T cells. IgG from ATLL could reduce the frequency of IL-4-producing CD4+ T cells, similarly to IgG from HAM/TSP /TSP, and could reduce the frequency of IFN-γ-producing γδT cells without influence on IL-17- and IL4-producing γδT and could reduce the frequency of IL-10- producing B cells. Finally, IgG from both HAM/TSP and ATLL patients could reduce the frequency of IFN-γ producing B cells. In conclusion, these results suggest that these preparations are active, partly overlapping in their effects, and able to elicit distinct effects on target populations.Copyright © 2023 Machado, Fagundes, Fernandes, Oliveira, Nukui, Casseb, Cunha, Nali, Sanabani and Victor.