为观察姜黄素对乳头状甲状腺癌B-CPAP细胞的迁移和侵袭的影响，我们将B-CPAP细胞分别用5、10、15或20μmol/L的姜黄素处理，并使用MTT测定法和Transwell测定法检测细胞存活、迁移和侵袭的变化。使用DCFH-DA探针检测处理后的细胞中的ROS水平。利用Western blotting和qRT-PCR测定细胞中Nrf2和Keap1的表达水平。姜黄素的处理剂量和时间依赖性地抑制了B-CPAP细胞的存活率（P < 0.05 或 P < 0.01）。姜黄素以剂量依赖方式抑制了B-CPAP细胞的迁移和侵袭（P < 0.001），并促进了ROS的产生，而应用NAC有效地逆转了姜黄素诱导的ROS增加。20μmol/L的姜黄素明显降低了Nrf2蛋白和mRNA的表达，并增加了Keap1蛋白和mRNA的表达（P < 0.05 或 P < 0.01），同时显著降低了细胞核中Nrf2蛋白的表达（P < 0.05），对细胞质中的Nrf2蛋白表达没有明显影响（P > 0.05）。姜黄素通过Keap1-Nrf2信号通路抑制了乳头状甲状腺癌B-CPAP细胞的增殖、迁移和侵袭。

To observe the effects of curcumin on migration and invasion of papillary thyriod cancer B-CPAP cells.B-CPAP cells were treated with 5, 10, 15, or 20 μmol/L curcumin, and the changes in cell survival, migration and invasion were examined using MTT assay and Transwell assay. ROS levels in the treated cells were detected with a DCFH-DA probe. The expression levels of Nrf2 and Keap1 in the cells were determined using Western blotting and qRT-PCR.Treatment with curcumin dose- and time-dependently suppressed the viability of B-CPAP cells (P < 0.05 or P < 0.01). Curcumin inhibited the migration and invasion (P < 0.001) and promoted ROS production in B-CPAP cells in a dose-dependent manner, and application of NAC effectively reversed curcumin- induced increase of ROS. Curcumin at 20 μmol/L significantly decreased the protein and mRNA expressions of Nrf2 and increased the expressions of Keap1 protein and mRNA (P < 0.05 or P < 0.01), causing also significantly reduced expression of Nrf2 protein in the cell nuclei (P < 0.05) without obviously affecting its expression in the cytoplasm (P > 0.05).Curcumin inhibits the proliferation, migration and invasion of papillary thyriod cancer B-CPAP cells probably via the Keap1-Nrf2 signaling pathway.