反复自发流产（RSA）对女性的生活质量有重大影响。了解流产背后的机制对于开发潜在的治疗方法至关重要。在各种流产模型中，CBA/J(♀) × DBA/2J(♂)模型作为研究最广泛的模型脱颖而出。该模型揭示了免疫系统改变对妊娠吸收的影响。白血病抑制因子（LIF）作为一种分泌性糖蛋白，在成功着床中具有重要作用。调节性T细胞（Tregs）在小鼠和人体内都能产生高水平的LIF。LIF通过上调表达Foxp3转录因子并下调RORγt表达，在Tregs的发育中起着重要作用。为了研究重组LIF（rLIF）对易流产（AP）小鼠的妊娠维持和Treg细胞频率的影响，本研究使用了一种特定的重组蛋白。AP组由CBA/J(♀) × DBA/2J(♂)小鼠组成，而对照组由CBA/J(♀) × BALB/c(♂)小鼠组成。在妊娠的第三天给AP组小鼠腹腔注射rLIF，并在此期间观察其对Treg细胞频率和妊娠维持的影响。在妊娠的第十四天注射rLIF后，AP小鼠的Foxp3表达显著增加（p = 0.02,0.008）。此外，注射rLIF的AP小鼠的吸收率显著降低（p = 0.01），出生率显著增加（p = 0.01,0.0005）。这些发现为LIF在治疗RSA患者中的潜在益处提供了新的见解。版权所有 © 2023 Elsevier B.V. 保留所有权利。

Recurrent spontaneous abortion (RSA) can have a significant impact on a woman's quality of life. Understanding the mechanisms behind abortion is crucial for developing potential treatments. Among various models of abortion, the CBA/J(♀) × DBA/2J(♂) model stands out as the most extensively studied. This model reveals the influence of an altered immune system on resorption during pregnancy. The leukemia inhibitory factor (LIF) holds considerable importance as a secretory glycoprotein essential for successful implantation. Regulatory T cells (Tregs) have been found to produce high levels of LIF in both mice and humans. LIF plays a vital role in the development of Tregs by upregulating the expression of the Foxp3 transcription factor while downregulating the expression of RORγt. To investigate the impact of recombinant LIF (rLIF) on pregnancy maintenance and Treg cell frequency in abortion-prone (AP) mice, a specific recombinant protein was used in this study. The AP group consisted of CBA/J(♀) × DBA/2J(♂) mice, while the control group comprised CBA/J(♀) × BALB/c(♂) mice. Intraperitoneal injections of rLIF were administered to the AP group on the third day of pregnancy, and its effects on Treg cell frequency and pregnancy maintenance were examined during this period. Following rLIF injections on the fourteenth day of pregnancy, the expression of Foxp3 significantly increased in AP mice (p = 0.02,0.008). Additionally, AP mice injected with rLIF demonstrated a significant reduction in resorption rate (p = 0.01) and a notable increase in birth rate (p = 0.01,0.0005). These findings provide new insights into the potential benefits of LIF in treating RSA patients.Copyright © 2023 Elsevier B.V. All rights reserved.