靶向氧化石墨烯（Graphene Oxide，GO）纳米颗粒能通过增加癌细胞靶向作用在癌症治疗中起重要作用。本研究旨在合成功能化壳聚糖-叶酸（chitosan-folate，CS-FA）修饰的GO纳米颗粒以输送一种天然产物罗孚森酮（Lawsone，LA）用于癌症治疗。经对LA-GO-CS-FA的表征后，通过ABTS、DPPH和FRAP测试研究了纳米颗粒的抗氧化活性。采用CAM试验研究了纳米颗粒对血管生成的影响。实时PCR评估了细胞经纳米颗粒处理后的炎症和血管生成基因表达水平。结果显示了纳米颗粒的形成，其尺寸为113.3 nm，PDI为0.31，表面电荷为+11.07 mV。封装效率的百分比报道为93%。与对照组相比，胃癌细胞对治疗最敏感，因此选择胃癌细胞研究基因表达变化。通过减少血管数量和长度以及减少CAM试验中胚胎的高度和重量来确认纳米颗粒的抗血管生成效果。实时PCR显示了涉及血管生成的基因的表达减少。通过抑制DPPH和ABTS自由基并在FRAP方法中减少铁离子，显示了纳米颗粒的高抗氧化性能。经纳米颗粒处理的AGS细胞减少了促炎基因的表达，表明纳米颗粒具有抗炎特性。本研究展示了纳米颗粒通过抑制血管生成来抑制胃癌细胞的功效。© 2023. 本作者等在版权所有，授予Springer Nature B.V.独家许可。

Targeted Graphene Oxide (GO) nanoparticles can play an important role in the treatment of cancer by increasing cancer cell targeting. This study was conducted to synthesize GO nanoparticles functionalized with chitosan-folate (CS-FA) to deliver a natural product Lawsone (LA) for cancer treatment.After characterization of the LA-GO-CS-FA, antioxidant activities of the nanoparticles were investigated by ABTS, DPPH, and FRAP tests. CAM assay was used to study the effect of nanoparticles on angiogenesis. The expression level of inflammatory and angiogenic genes in cells treated with nanoparticles was evaluated by real-time PCR.The findings demonstrated the formation of nanoparticles with a size of 113.3 nm, a PDI of 0.31, and a surface charge of + 11.07 mV. The percentages of encapsulation efficiency were reported at 93%. Gastric cancer cells were reported as the most sensitive to treatment compared to the control, and the gastric cancer cells were used to study gene expression changes. The anti-angiogenic effects of nanoparticles were confirmed by reducing the average number and length of blood vessels and reducing the height and weight of embryos in the CAM assay. The reducing the expression of genes involved in angiogenesis in real-time PCR was demonstrated. Nanoparticles displayed high antioxidant properties by inhibiting DPPH and ABTS radicals and reducing iron ions in the FRAP method. The reduction of pro-inflammatory genes in AGS cells which were treated with nanoparticles indicates the anti-inflammatory properties of nanoparticles.This study showed the efficacy of nanoparticles in inhibiting gastric cancer cells by relying on inhibiting angiogenesis.© 2023. The Author(s), under exclusive licence to Springer Nature B.V.