本研究调查了黑色马先蒿（Gymnadenia nigra Rchb.f.同义词Nigritella nigra (L.) Rchb. f.）（兰科）的地上部分（GNAR）和地下部分（GNUG）的生物活性。当样品在两种肾上腺皮质癌细胞系（SW13和H295R）中进行MTT试验和尝试蓝染色排斥试验时，所得数据令人感兴趣。特别是，GNAR 80% 甲醇提取物在1µg/µL浓度下，在24小时后明显抑制了细胞存活率，细胞形态评估结果也显示出显著结果，特别是在两种提取物的作用下SW13细胞。GNAR提取物较GNUG提取物更能清除DPPH自由基，它在白蛋白变性（最大变性百分比463.0 ± 8.3 vs 77.3 ± 13.3）和蛋白酶抑制（最大抑制百分比138.5 ± 7.0 vs 2.1 ± 2.0）测试中也更活跃。这些结果强调了黑色马先蒿在体外的重要抗肿瘤活性，值得进一步研究。

This study investigated the bioactivity of both aerial (GNAR) and underground (GNUG) parts of Gymnadenia nigra Rchb.f. (syn. Nigritella nigra (L.) Rchb. f.) (Orchidaceae). The obtained data proved interesting when the samples were tested in two adrenocortical cancer cell lines (SW13 and H295R). In particular, the GNAR 80% methanol extract distinctly inhibited their viability after 24 h at a concentration of 1 µg/µL by MTT assay and trypan blue dye exclusion method. Cell morphology evaluation by means Wright's staining also showed significant results, particularly in SW13 cells under the effect of both extracts. GNAR extract was able to scavenge the DPPH radical better than GNUG extract. It also was more active in albumin denaturation (a maximum % denaturation equal to 463.0 ± 8.3 vs 77.3 ± 13.3) and protease inhibition (a maximum % inhibition equal to 138.5 ± 7.0 vs 2.1 ± 2.0) tests. The results highlighted an important antitumor activity of G. nigra in vitro that deserves to be further studied.