一旦发生核事故，应迅速识别受到高剂量辐射（>1-2 Gy）的个人。虽然铁氧还蛋白还原酶（FDXR）最近被建议作为辐射响应基因，但单基因生物标志物的使用限制了辐射剂量评估。为了克服这一限制，我们寻求鉴定可靠的辐射响应基因生物标志物。在全身照射后从小鼠体内分离外周血单核细胞（PBMC），并使用微阵列方法分析基因表达，以鉴定辐射响应基因。为了了解辐射暴露后免疫反应的重要作用，我们选择了小鼠和人类 PBMC 中受辐射暴露上调的几种免疫相关候选基因。特别是，ACOD1 和 CXCL10 的表达以辐射剂量依赖性方式增加，而在人 PBMC 中脂多糖 (LPS) 刺激后保持不变。进一步评估了放疗前后癌症患者血液中这两种基因的表达。放疗后CXCL10表达量明显增加，且与FDXR表达呈正相关。受辐射PBMC中CXCL10表达量代表了放射暴露的潜在生物标志物。

In case of a nuclear accident, individuals with high-dose radiation exposure (>1-2 Gy) should be rapidly identified. While ferredoxin reductase (FDXR) was recently suggested as a radiation-responsive gene, the use of a single gene biomarker limits radiation dose assessment. To overcome this limitation, we sought to identify reliable radiation-responsive gene biomarkers.Peripheral blood mononuclear cells (PBMCs) were isolated from mice after total body irradiation, and gene expression was analyzed using a microarray approach to identify radiation-responsive genes.In light of the essential role of the immune response following radiation exposure, we selected several immune-related candidate genes upregulated by radiation exposure in both mouse and human PBMCs. In particular, the expression of ACOD1 and CXCL10 increased in a radiation dose-dependent manner, while remaining unchanged following lipopolysaccharide (LPS) stimulation in human PBMCs. The expression of both genes was further evaluated in the blood of cancer patients before and after radiotherapy. CXCL10 expression exhibited a distinct increase after radiotherapy and was positively correlated with FDXR expression.CXCL10 expression in irradiated PBMCs represents a potential biomarker for radiation exposure.