SUMO 化是一种翻译后修饰，涉及小泛素样修饰剂 (SUMO) 蛋白与靶底物的共价缀合，调节各种重要的分子和细胞过程，包括转录、细胞周期、细胞信号传导和 DNA 合成和修复。新合成的 SUMO 尚未成熟，会被 SUMO 特异性蛋白酶家族裂解，导致 C 端 Gly-Gly 基序暴露，从而成为成熟形式。在 ATP 存在的情况下，成熟的 SUMO 通过 E1 的半胱氨酸残基与激活酶 E1 缀合，然后转移到人体内 E2 缀合酶 Ubc9 的半胱氨酸残基，该酶识别并修饰底物蛋白的赖氨酸残基。 E3 SUMO 连接酶促进 SUMO 化。 SUMO 化是一种可逆修饰，由 SUMO 特异性蛋白酶介导。累积研究表明 SUMO 化以多种方式影响蛋白质底物的功能，包括细胞定位和蛋白质稳定性。小鼠基因敲除研究表明，几种 SUMO 循环机械蛋白对于包括免疫细胞在内的各种细胞谱系的发育和分化至关重要。异常的 SUMO 化与多种疾病有关，包括癌症、心血管疾病和自身免疫性疾病。本综述总结了 SUMO 修饰的生物化学以及参与 SUMO 化的蛋白质的一般生物学功能。本综述特别关注 SUMO 化调节免疫细胞（包括 T、B、树突状细胞和骨髓细胞）发育、成熟和功能的分子机制。本综述还讨论了 SUMO 循环破坏和疾病（包括癌症和传染病）免疫细胞中靶蛋白 SUMO 化的位点特异性中断的潜在相关性。© 2024。作者。

SUMOylation, which is a type of post-translational modification that involves covalent conjugation of small ubiquitin-like modifier (SUMO) proteins to target substrates, regulates various important molecular and cellular processes, including transcription, the cell cycle, cell signaling, and DNA synthesis and repair. Newly synthesized SUMO is immature and cleaved by the SUMO-specific protease family, resulting in exposure of the C-terminal Gly-Gly motif to become the mature form. In the presence of ATP, mature SUMO is conjugated with the activating enzyme E1 through the cysteine residue of E1, followed by transfer to the cysteine residue of E2-conjugating enzyme Ubc9 in humans that recognizes and modifies the lysine residue of a substrate protein. E3 SUMO ligases promote SUMOylation. SUMOylation is a reversible modification and mediated by SUMO-specific proteases. Cumulative studies have indicated that SUMOylation affects the functions of protein substrates in various manners, including cellular localization and protein stability. Gene knockout studies in mice have revealed that several SUMO cycling machinery proteins are crucial for the development and differentiation of various cell lineages, including immune cells. Aberrant SUMOylation has been implicated in several types of diseases, including cancers, cardiovascular diseases, and autoimmune diseases. This review summarizes the biochemistry of SUMO modification and the general biological functions of proteins involved in SUMOylation. In particular, this review focuses on the molecular mechanisms by which SUMOylation regulates the development, maturation, and functions of immune cells, including T, B, dendritic, and myeloid cells. This review also discusses the underlying relevance of disruption of SUMO cycling and site-specific interruption of SUMOylation on target proteins in immune cells in diseases, including cancers and infectious diseases.© 2024. The Author(s).