B 细胞特异性莫洛尼鼠白血病病毒插入位点 1 (BMI-1) 的上调与许多癌症的侵袭、转移和不良预后有关。本研究旨在评估唾液腺腺样囊性癌（SACC）患者唾液中BMI-1的水平及临床意义，分析BMI-1在SACC侵袭转移中的生物学功能及机制。测定SACC患者唾液和肿瘤组织中BMI-1的水平。分析SACC患者唾液BMI-1水平与临床病理参数和临床结局的相关性。此外，通过外源性过表达和沉默 BMI，研究了 BMI-1 对体外伤口愈合、Transwell 侵袭和上皮间质转化 (EMT) 相关蛋白表达以及体内致瘤性和实验性肺转移的影响SACC细胞中BMI-1为-1。侵袭或转移的SACC患者唾液和肿瘤组织中BMI-1水平升高。高唾液 BMI-1 水平与较差的 TNM 分期、较差的总生存率和无病生存率相关。 SACC-83中BMI-1的外源表达促进其迁移和侵袭，而SACC-LM中BMI-1的沉默可抑制其体外迁移和侵袭，并抑制体内肿瘤发生和肺转移。此外，BMI-1调节SACC中EMT相关蛋白的表达。我们的研究表明，BMI-1可以作为有价值的生物标志物来识别SACC中的肿瘤侵袭和转移，预测其预后，并作为有前途的治疗靶点SACC.© 2023 中华民国齿科医学会。 Elsevier B.V. 的出版服务

Upregulation of B-cell specific Moloney murine leukemia virus insertion site 1 (BMI-1) has been involved in the invasion, metastasis, and poor prognosis of many cancers. The aim of this study was to evaluate the levels and clinical significance of BMI-1 in saliva of patients with salivary adenoid cystic carcinoma (SACC), and to analyze biological function and mechanism of BMI-1 in the invasion and metastasis of SACC.The levels of BMI-1 in saliva and tumor tissues of SACC patients were determined. The correlation of salivary BMI-1 levels with clinicopathological parameters and clinical outcomes in patients with SACC was analyzed. Additionally, the effects of BMI-1 on wound-healing, transwell invasion, and epithelial-mesenchymal transition (EMT)-related protein expression in vitro as well as on tumorigenicity and experimental lung metastasis in vivo were investigated through exogenous overexpression and silencing of BMI-1 in SACC cells.BMI-1 levels increased in saliva and tumor tissues in SACC patients with invasion or metastasis. High salivary BMI-1 levels were correlated with poor TNM stage, poor overall survival, and disease-free survival. Exogenous expression of BMI-1 in SACC-83 promoted its migration and invasion, while silencing BMI-1 in SACC-LM inhibited its migration and invasion in vitro and suppressed tumorigenesis and lung metastasis in vivo. Furthermore, BMI-1 regulated the expression of EMT-related proteins in SACC.Our study shows that BMI-1 can serve as a valuable biomarker to identify tumor invasion and metastasis in SACC, predict its prognosis, and act as a promising therapeutic target for SACC.© 2023 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.