目前的研究采用沉淀法制备纯钙羟基磷灰石（HA）和铈取代羟基磷灰石（Ce-HA）纳米粒子，其中铈离子以3至7wt%的不同浓度交换成HA结构。 X 射线粉末衍射 (XRD)、场发射扫描电子显微镜 (FE-SEM)、高分辨率透射电子显微镜 (HR-TEM)、傅立叶变换红外 (FTIR) 光谱、Brunauer-Emmett-Teller (BET) 表面积测量和zeta电位用于检查纳米颗粒的结构特征。此外，还研究了所生产的材料对革兰氏阳性、革兰氏阴性和真菌细菌种类的抗菌和抗真菌作用。掺杂铈的纳米粒子显示出有效的抗菌和抗真菌特性。所有样品均在模拟体液 (SBF) 中进行生物活性测试，并使用 SEM 结合能量色散 X 射线 (EDX) 突出显示其表面磷灰石层的形成。Ce-HA 中释放的阿霉素 (DOX)纳米粒子和纯 HA 在磷酸盐缓冲盐水 (PBS) 中进行长达 28 天的测试。两种纳米颗粒都能够在半满载的情况下释放药物。同样，评估了所有生产的样品对MG-63细胞系的细胞毒作用，所有样品均表现出良好的细胞相容性。负载阿霉素的纳米粒子的细胞毒作用显示出对骨癌细胞的有希望的抗癌活性，特别是对于高铈含量的样品。由此产生的纳米颗粒显示出将阿霉素递送至骨癌并具有骨再生能力的出色前景。版权所有 © 2024。由 Elsevier B.V. 出版。

The current study used the precipitation method to prepare pure calcium hydroxyapatite (HA) and cerium-substituted hydroxyapatite (Ce-HA) nanoparticles, where cerium ions were exchanged into the HA structure at different concentrations ranging from 3 to 7 wt%. X-ray powder diffraction (XRD), field emission scanning electron microscopy (FE-SEM), high resolution transmission electron microscopy (HR-TEM), Fourier transform infrared (FTIR) spectroscopy, Brunauer-Emmett-Teller (BET) surface area measurements, and zeta potential were used to examine the structural characteristics of the nanoparticles. Additionally, the antibacterial and antifungal effects of the produced materials on Gram-positive, Gram-negative, and fungal bacterial species were studied. Nanoparticles with cerium doping showed effective antibacterial and antifungal properties. All samples were tested for bioactivity in simulated body fluid (SBF), and the formation of an apatite layer on their surfaces was highlighted using SEM in conjunction with energy-dispersive X-rays (EDX).Doxorubicin (DOX) release from Ce-HA nanoparticles and pure HA was tested in phosphate-buffered saline (PBS) for up to 28 days. Both nanoparticles were able to release the drug while still being semi-fully loaded. Similarly, the cytotoxic effect of all produced samples on the MG-63 cell line was evaluated, and all samples showed good cytocompatibility. The cytotoxic effect of doxorubicin-loaded nanoparticles showed promising anticancer activity against bone cancer cells, especially samples with high cerium content. The resulting nanoparticles show excellent promising ability for the delivery of doxorubicin to bone cancer with the capacity for bone regeneration.Copyright © 2024. Published by Elsevier B.V.