肠道病毒C116（EV-C116）是肠道病毒C组的新成员，与多种传染病密切相关。尽管零星研究已在世界各地的临床样本中检测到 EV-C116，但目前可用的信息有限。在这项研究中，通过宏转录组测序，在表面健康的儿童中检测到了两份 EV-C 阳性粪便样本，其中丰度较低。基于几个 EV-C 的原型，观察到了两个谱系。谱系1包括许多在细胞培养中不会引起EV样细胞病变效应的类型。 EV-C116 在最大似然树中分为三个基因组，本研究中的两个菌株（XZ2 和 XZ113）形成了两个不同的谱系，表明 EV-C116 仍然在全球范围内扩散。在 XZ2 菌株中观察到明显的型间重组事件，其中 CVA22 被确定为次要供体。然而，另一种菌株（XZ113）经历了不同的重组情况，凸显了重组在EV-Cs生物多样性形成中的重要性。 EV-C116 菌株可以在横纹肌肉瘤细胞培养物中以低滴度繁殖；然而，没有观察到类似 EV 的细胞病变效应。 HEp-2、L20B、VERO 和 293T 细胞系没有为 EV-C116 生长提供适当的环境。这些结果挑战了对 EV-C116 菌株非培养性质的传统认识，并解释了临床检测的困难。© 2024 Wiley periodicals LLC。

Enterovirus C116 (EV-C116) is a new member of the enterovirus C group which is closely associated with several infectious diseases. Although sporadic studies have detected EV-C116 in clinical samples worldwide, there is currently limited information available. In this study, two EV-C-positive fecal specimens were detected in apparently healthy children, which harbored low abundance, through meta-transcriptome sequencing. Based on the prototypes of several EV-Cs, two lineages were observed. Lineage 1 included many types that could not cause EV-like cytopathic effect in cell culture. Three genogroups of EV-C116 were divided in the maximum likelihood tree, and the two strains in this study (XZ2 and XZ113) formed two different lineages, suggesting that EV-C116 still diffuses worldwide. Obvious inter-type recombination events were observed in the XZ2 strain, with CVA22 identified as a minor donor. However, another strain (XZ113) underwent different recombination situations, highlighting the importance of recombination in the formation of EV-Cs biodiversity. The EV-C116 strains could propagate in rhabdomyosarcoma cell cultures at low titer; however, EV-like cytopathic effects were not observed. HEp-2, L20B, VERO, and 293T cell lines did not provide an appropriate environment for EV-C116 growth. These results challenge the traditional recognition of the uncultured nature of EV-C116 strains and explain the difficulty of clinical detection.© 2024 Wiley Periodicals LLC.