对黄杨树枝和叶子的甲醇提取物进行了广泛的植物化学研究，鉴定出 41 种黄杨生物碱，其中包括 20 种未描述的生物碱，即环黄杨素 A-I (1-7、16 和 20)，以及次黄杨素 A-K (8 -15 和 17-19)。它们的结构是通过使用各种光谱技术进行详细分析来描绘的。 cyclobuxusinines B (2)是第一个黄杨生物碱，其CH3-18被氧化，暗示该植物中存在特殊的氧化酶。 Secobuxusinines C (10)、D (11) 和 E (12) 的 C-12 或 C-19 具有 OH 基团取代，丰富了黄杨生物碱中的取代基模式，并表明黄杨属生物碱的结构更加多样化。在对其生物活性的评估中，其中一些对两种人类肿瘤卵巢癌细胞系表现出显着的细胞毒性作用。值得注意的是，化合物 36 对 ES2 和 A2780 细胞系表现出更有效的细胞毒作用，IC50 值分别为 1.33 μM 和 0.48 μM。版权所有 © 2024。由 Elsevier Ltd 出版。

Extensive phytochemical study of the methanol extract of twigs and leaves of Buxus sinica resulted in the identification of forty-one Buxus alkaloids, including twenty undescribed ones, namely cyclobuxusinines A-I (1-7, 16 and 20), as well as secobuxusinines A-K (8-15 and 17-19). Their structures were delineated by detailed analysis using various spectroscopic techniques. cyclobuxusinines B (2) was the first Buxus alkaloid, whose CH3-18 was oxidized, implying the presence of special oxidative enzymes in this plant. Secobuxusinines C (10), D (11), and E (12), whose C-12 or C-19 have an OH group substitution, enriched the substituent pattern in Buxus alkaloid and suggested more structurally diverse alkaloids in the Buxus spp. In the assessment of their bioactivities, some of them exhibited significant cytotoxic effects on two human tumor ovarian cancer cell lines. Notably, compound 36 displayed more potent cytotoxic effect against ES2 and A2780 cell lines with the IC50 value of 1.33 μM and 0.48 μM, respectively.Copyright © 2024. Published by Elsevier Ltd.