本研究的目的是确定组织学正常的胆囊结石的炎症特征，并比较急性和慢性炎症胆囊中炎症标志物的表达。对福尔马林固定石蜡包埋的胆囊进行免疫组织化学检测肿瘤坏死因子（TNF）- α、白介素 (IL)-6、IL-2R 和 p 物质分三组：第一组 (n = 60) 慢性胆囊炎、第二组 (n = 57) 急性胆囊炎和第三组 (n = 45) 组织学正常胆囊患有胆结石。使用H评分系统对表达进行定量。与III组（0.40，0.10）相比，I组（2.65，0.87-7.97）和II组（12.30，6.15-25.55）中粘膜IL-2R的中位四分位数范围表达显着增加-1.35，p < 0.05）。与 III 组（0.50、0.15-1.05，p < 0.05）相比，I 组（2.0、1.12-4.95）和 II 组（10.0、5.95-14.30）粘膜下 IL-2R 表达也显着增加。 I组（5.95，1.60-18.15）、II组（6.10，1.1-36.15）和III组（8.30，2.60-26.35，p>0.05）之间淋巴细胞IL-6表达没有差异。与第 I 组（0.5、0-10.2，p < 0.05）相比，第 III 组的上皮 IL-6 表达（8.3、2.6-26.3）显着增加，第 III 组的上皮 TNF-α 表达也显着增加（85.0、70.50-92.0）与第一组（72.50、45.25.0-85.50，p < 0.05）和第二组（61.0、30.0-92.0，p < 0.05）相比。与第 III 组 (1.0,1.0-1.30, p < 0.05) 相比，第 I 组 (1.90, 1.32-2.65) 和 II 组 (5.62, 2.50-20.8) 中的淋巴细胞 P 物质表达显着增加。与 I 组（75.7、50.6-105.3，p < 0.05）和 II 组（78.9、43.5-118.5，p < 0.05）相比，III 组中 P 物质的上皮细胞表达显着增加。组织学正常患有胆结石的胆囊在免疫组织化学上表现出炎症特征。© 2024 作者。约翰·威利出版的《世界外科杂志》

The aim of this study was to establish features of inflammation in histologically normal gallbladders with gallstones and compare the expression of inflammatory markers in acutely and chronically inflamed gallbladders.Immunohistochemistry was performed on formalin-fixed paraffin-embedded gallbladders for tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-2R, and substance p in three groups: Group I (n = 60) chronic cholecystitis, Group II (n = 57) acute cholecystitis and Group III (n = 45) histologically normal gallbladders with gallstones. Expression was quantified using the H-scoring system.Median, interquartile range expression of mucosal IL-2R in Groups I (2.65, 0.87-7.97) and II (12.30, 6.15-25.55) was significantly increased compared with group III (0.40, 0.10-1.35, p < 0.05). Submucosal IL-2R expression in Groups I (2.0, 1.12-4.95) and II (10.0, 5.95-14.30) was also significantly increased compared with Group III (0.50, 0.15-1.05, p < 0.05). There was no difference in the lymphoid cell IL-6 expression between Groups I (5.95, 1.60-18.15), II (6.10, 1.1-36.15) and III (8.30, 2.60-26.35, p > 0.05). Epithelial IL-6 expression of Group III (8.3, 2.6-26.3) was significantly increased compared with group I (0.5, 0-10.2, p < 0.05) as was epithelial TNF-α expression in Group III (85.0, 70.50-92.0) compared with Groups I (72.50, 45.25.0-85.50, p < 0.05) and II (61.0, 30.0-92.0, p < 0.05). Lymphoid cell Substance P expression in Groups I (1.90, 1.32-2.65) and II (5.62, 2.50-20.8) was significantly increased compared with Group III (1.0,1.0-1.30, p < 0.05). Epithelial cell expression of Substance P in Group III (121.7, 94.6-167.8) was significantly increased compared with Groups I (75.7, 50.6-105.3, p < 0.05) and II (78.9, 43.5-118.5, p < 0.05).Histologically normal gallbladders with gallstones exhibited features of inflammation on immunohistochemistry.© 2024 The Author(s). World Journal of Surgery published by John Wiley & Sons Ltd on behalf of International Society of Surgery/Société Internationale de Chirurgie (ISS/SIC).