萝卜硫素（SFN）对包括胃癌在内的多种癌症具有抗癌作用。然而，SFN 对程序性死亡配体 1 (PD-L1) 和胃癌检查点阻断治疗的调节作用尚未阐明。在这里，我们在体外和体内研究中证明了 SFN 抑制胃癌细胞的生长。 SFN 通过激活胃癌细胞中的 ΔNP63α 上调 PD-L1 表达。此外，我们发现SFN削弱了抗PD-L1单克隆抗体（α-PD-L1 mab）对胃癌细胞的抗癌作用。这些结果揭示了一种新的 PD-L1 调节机制以及 SFN 在胃癌干预中的双刃作用。© 2024 Wiley periodicals LLC。

Sulforaphane (SFN) exerts anticancer effect on various cancers including gastric cancer. However, the regulatory effect of SFN on programmed death-ligand 1 (PD-L1) and checkpoint blockade therapy in gastric cancer have not been elucidated. Here we demonstrated that SFN suppressed gastric cancer cell growth both in vitro and in vivo study. SFN upregulated PD-L1 expression through activating ΔNP63α in gastric cancer cells. Further, we found that SFN impaired the anticancer effect of anti-PD-L1 monoclonal antibody (α-PD-L1 mab) on gastric cancer cells. These results uncover a novel PD-L1 regulatory mechanism and the double-edged role of SFN in gastric cancer intervention.© 2024 Wiley Periodicals LLC.