棒藻属八珊瑚是一种海洋无脊椎动物，具有丰富的细胞毒性次生代谢产物，如前列腺素类和多拉拉烷类。在我们对 C. spp. 的连续天然产物研究中，鉴定出了两种先前未描述的前列腺素类化合物 [克拉维酮 I-15-one (1) 和 12-O-去乙酰克拉维酮 I (2)] 和 11 种已知类似物 (3-13)。通过一维和二维核磁共振、高分辨质谱和红外数据分析，阐明了这些新化合物的结构。此外，所有测试的前列腺素类药物（1 和 3-13）均对人类口腔癌细胞系 (Ca9-22) 表现出有效的细胞毒活性。主要化合物 3 对 Ca9-22 细胞表现出细胞毒活性，IC50 值为 2.11 ± 0.03 μg/mL，与珊瑚提取物的细胞毒作用相呼应。此外，计算机工具还用于预测分离化合物对人类肿瘤细胞系和一氧化氮产生的可能影响，以及药理学潜力。

Octocoral of the genus Clavularia is a kind of marine invertebrate possessing abundant cytotoxic secondary metabolites, such as prostanoids and dolabellanes. In our continuous natural product study of C. spp., two previously undescribed prostanoids [clavulone I-15-one (1) and 12-O-deacetylclavulone I (2)] and eleven known analogs (3-13) were identified. The structures of these new compounds were elucidated based on analysis of their 1D and 2D NMR, HRESIMS, and IR data. Additionally, all tested prostanoids (1 and 3-13) showed potent cytotoxic activities against the human oral cancer cell line (Ca9-22). The major compound 3 showed cytotoxic activity against the Ca9-22 cells with the IC50 value of 2.11 ± 0.03 μg/mL, which echoes the cytotoxic effect of the coral extract. In addition, in silico tools were used to predict the possible effects of isolated compounds on human tumor cell lines and nitric oxide production, as well as the pharmacological potentials.