软组织肉瘤（STS）是一种相对罕见的恶性肿瘤，约占所有成人癌症的 1%。已知它有 70 多种亚型。它的稀有性，加上其不同的亚型，使得早期诊断具有挑战性。目前 STS 的标准治疗方法是手术切除。为了确定 STS 的预后和病理生理学，我们对 24 名接受手术肿瘤切除的 STS 患者的术前和术后血浆样本进行了非靶向代谢分析。使用超高效液相色谱-四极杆飞行时间/质谱法进行分析。鉴定出 39 种推定代谢物，包括磷脂和酰基肉碱，表明脂质代谢发生变化。术后样本中磷脂含量增加，而酰基肉碱含量减少。值得注意的是，术后复发的患者术前溶血磷脂酰胆碱 (LPC) O-18:0 和 LPC O-16:2 水平显着低于未复发的患者。代谢分析可以识别对脂质合酶抑制剂敏感的侵袭性肿瘤。我们相信，这些发现可能有助于阐明 STS 的病理生理学，并有助于开展这种罕见恶性肿瘤的进一步代谢研究。

Soft tissue sarcoma (STS) is a relatively rare malignancy, accounting for about 1% of all adult cancers. It is known to have more than 70 subtypes. Its rarity, coupled with its various subtypes, makes early diagnosis challenging. The current standard treatment for STS is surgical removal. To identify the prognosis and pathophysiology of STS, we conducted untargeted metabolic profiling on pre-operative and post-operative plasma samples from 24 STS patients who underwent surgical tumor removal. Profiling was conducted using ultra-high-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry. Thirty-nine putative metabolites, including phospholipids and acyl-carnitines were identified, indicating changes in lipid metabolism. Phospholipids exhibited an increase in the post-operative samples, while acyl-carnitines showed a decrease. Notably, the levels of pre-operative lysophosphatidylcholine (LPC) O-18:0 and LPC O-16:2 were significantly lower in patients who experienced recurrence after surgery compared to those who did not. Metabolic profiling may identify aggressive tumors that are susceptible to lipid synthase inhibitors. We believe that these findings could contribute to the elucidation of the pathophysiology of STS and the development of further metabolic studies in this rare malignancy.