空肠弯曲杆菌是一种人畜共患细菌，在致病过程中能够侵入上皮细胞。已经确定了几种细菌因素有助于这一过程，但我们对宿主反应的了解仍然非常有限。为了揭示这种反应的主要途径，我们进行了全转录组分析 (WTA)，比较了 INT407 上皮细胞内化第 1 小时和第 3 小时之间的基因表达。在测试的41,769个人类基因中，WTA显示有19,060个基因受到不同程度的影响。转录(296/1052; 28%)、信号转导(215/1052; 21%)、细胞凋亡(153/1052; 15%)、免疫反应(97/1052; 9%)、跨膜运输的基因和调控因子（64/1052；6%）、细胞间信号传导（32/1052；3%）、细胞间粘附（29/1052；3%）和碳水化合物代谢（28/1052；3%）受影响最大生物学功能。内化过程这一阶段基因表达的一个显着特征是免疫功能和细胞凋亡的激活，这令人信服地概述了被入侵的细胞面临死亡和生存的选择。入侵者和宿主之间看似平衡的现状是一个复杂过程的结果，该过程也影响已知与感染后病理状况相关的基因。两种一般肿瘤标志物 TLR3 (3.79×) 和 CD36 (2.73×) 以及肿瘤发生的三个因素 SERPINEB9 (11.37×)、FNDC1 (7.58×) 和 TACR2 (8.84×) 的上调证实了更广泛的病理意义这种细菌。

Campylobacter jejuni is a zoonotic bacterium with the capacity to invade the epithelial cells during the pathogenic process. Several bacterial factors have been identified to contribute to this process, but our knowledge is still very limited about the response of the host. To reveal the major routes of this response, a whole-transcriptome analysis (WTA) was performed where gene expressions were compared between the 1st and the 3rd hours of internalization in INT407 epithelial cells. From the 41,769 human genes tested, altogether, 19,060 genes were shown through WTA to be influenced to different extents. The genes and regulation factors of transcription (296/1052; 28%), signal transduction (215/1052; 21%), apoptosis (153/1052; 15%), immune responses (97/1052; 9%), transmembrane transport (64/1052; 6%), cell-cell signaling (32/1052; 3%), cell-cell adhesions (29/1052; 3%), and carbohydrate metabolism (28/1052; 3%) were the most affected biological functions. A striking feature of the gene expression of this stage of the internalization process is the activation of both immune functions and apoptosis, which convincingly outlines that the invaded cell faces a choice between death and survival. The seemingly balanced status quo between the invader and the host is the result of a complex process that also affects genes known to be associated with postinfectious pathological conditions. The upregulation of TLR3 (3.79×) and CD36 (2.73×), two general tumor markers, and SERPINEB9 (11.37×), FNDC1 (7.58×), and TACR2 (8.84×), three factors of tumorigenesis, confirms the wider pathological significance of this bacterium.