关于游离脂肪酸受体 2 (FFAR2) 蛋白表达的信息很少，尤其是在肿瘤中。因此，本研究的目的是利用免疫组织化学全面表征大量人类正常和肿瘤组织中 FFAR2 的表达谱，从而为进一步深入研究其潜在的诊断或治疗重要性提供基础。新型兔多克隆抗 FFAR2 抗体 0524，针对人 FFAR2 的 C 末端区域。使用表达 FFAR2 的细胞系 BON-1 和 FFAR2 特异性小干扰 RNA 以及天然和 FFAR2 转染的 HEK-293 细胞，通过蛋白质印迹分析和免疫细胞化学证实了抗体特异性。然后将该抗体用于对各种福尔马林固定、石蜡包埋的正常和肿瘤人体组织标本进行免疫组织化学分析。在正常组织中，FFAR2 主要存在于大脑皮层、滤泡细胞和甲状腺 C 细胞的不同细胞群中、心脏的心肌细胞、支气管上皮和腺体、肝脏的肝细胞和胆管上皮、胆囊上皮、内分泌胰腺的外分泌和β细胞、肾小球系膜细胞和足细胞以及肾的集合管、肠粘膜（特别是肠内分泌细胞）、前列腺上皮、睾丸曲细精管和胎盘合体滋养细胞。在肿瘤组织中，FFAR2 在甲状腺乳头状癌、甲状旁腺腺瘤以及胃癌、结肠癌、胰腺癌、肝细胞癌、胆管细胞癌、膀胱癌、乳腺癌、宫颈癌和卵巢癌中尤其普遍。我们生成并表征了一种新型兔多克隆抗人 FFAR2该抗体非常适合可视化人类常规病理组织中的 FFAR2 表达。该抗体也适用于蛋白质印迹和免疫细胞化学实验。据我们所知，该抗体在各种正常和肿瘤性人体组织中首次实现了广泛的 FFAR2 蛋白表达谱。版权所有 © 2023。由 Elsevier Inc. 出版。

Little information is available concerning protein expression of the free fatty acid receptor 2 (FFAR2), especially in tumours. Therefore, the aim of the present study was to comprehensively characterise the expression profile of FFAR2 in a large series of human normal and neoplastic tissues using immunohistochemistry thus providing a basis for further in-depth investigations into its potential diagnostic or therapeutic importance.We developed a novel rabbit polyclonal anti-FFAR2 antibody, 0524, directed against the C-terminal region of human FFAR2. Antibody specificity was confirmed via Western blot analyses and immunocytochemistry using the FFAR2-expressing cell line BON-1 and FFAR2-specific small interfering RNA as well as native and FFAR2-transfected HEK-293 cells. The antibody was then used for immunohistochemical analyses of various formalin-fixed, paraffin-embedded specimens of normal and neoplastic human tissues.In normal tissues, FFAR2 was mainly present in distinct cell populations of the cerebral cortex, follicular cells and C cells of the thyroid, cardiomyocytes of the heart, bronchial epithelia and glands, hepatocytes and bile duct epithelia of the liver, gall bladder epithelium, exocrine and β-cells of the endocrine pancreas, glomerular mesangial cells and podocytes as well as collecting ducts of the kidney, intestinal mucosa (particularly enteroendocrine cells), prostate epithelium, seminiferous tubules of the testicles, and placental syncytiotrophoblasts. In neoplastic tissues, FFAR2 was particularly prevalent in papillary thyroid carcinomas, parathyroid adenomas, and gastric, colon, pancreatic, hepatocellular, cholangiocellular, urinary bladder, breast, cervical, and ovarian carcinomas.We generated and characterised a novel rabbit polyclonal anti-human FFAR2 antibody that is well-suited for visualising FFAR2 expression in human routine pathology tissues. This antibody is also suitable for Western blot and immunocytochemistry experiments. To our knowledge, this antibody enabled the first broad FFAR2 protein expression profile in various normal and neoplastic human tissues.Copyright © 2023. Published by Elsevier Inc.