随着纳米技术的快速发展，各种功能纳米材料在药物输送、抗肿瘤、抗菌治疗等生物医学领域显示出令人兴奋的潜力。这些纳米材料提高了负载药物的稳定性和选择性，减少药物引起的副作用，实现药物的控制和靶向释放，提高治疗效果。医疗实践中抗真菌杀菌剂耐药性的增加及其副作用激发了人们对新疗法的兴趣，例如光动力疗法（PDT），该疗法不会在微生物中产生耐药性并有效控制病理学。本研究旨在体外评估使用 1,9-二甲基亚甲基蓝 (DMMB) 作为光敏剂、红色 LED (λ630) 和 DMMB 纳米封装 (RL-NPs/DMMB) 进行光动力疗法对白色念珠菌的疗效使用铜绿假单胞菌产生的鼠李糖脂，通过 DMMB RL 的表征和菌落形成计数来评估 DMMB RL 颗粒是否比单独的 DMMB 具有更好的性能。测试在六个实验组（对照组、DMMB、RL-NPs、RL-NPs/DMMB、PDT 和 PDT RL-NPs/DMMB）中进行，使用纳米粒子、用鼠李糖脂封装的 DMMB (750 ng/mL)光源采用 1:1 比例的原型，由一组能量密度为 20 J/cm2 的红色 LED 组成。结果表明，与传统应用相比，应用 PDT 结合封装 (RL-NPs/DMMB) 是一种更实用的抑制白色念珠菌的方法（减少 2 个对数），并具有可能的临床应用方案。版权所有 © 2024 Elsevier B.V. 保留所有权利。

With the rapid development of nanotechnology, various functional nanomaterials have shown exciting potential in biomedical areas such as drug delivery, antitumor, and antibacterial therapy. These nanomaterials improve the stability and selectivity of loaded drugs, reduce drug-induced side effects, realize controlled and targeted drug release, and increase therapeutic efficacy. The increased resistance to antifungal microbicides in medical practice and their side effects stimulate interest in new therapies, such as Photodynamic Therapy (PDT), which do not generate resistance in microorganisms and effectively control the pathology. The present study aimed to evaluate, in vitro, the efficacy of photodynamic therapy on Candida albicans using 1,9-Dimethyl-Methylene Blue (DMMB) as photosensitizer, red LED (λ630), and nanoencapsulation of DMMB (RL-NPs/DMMB) using rhamnolipids produced by Pseudomonas aeruginosa to evaluate if there is better performance of DMMB + RL particles compared to DMMB alone via the characterization of DMMB + RL and colony forming count. The tests were carried out across six experimental groups (Control, DMMB, RL-NPs, RL-NPs/DMMB, PDT and PDT + RL-NPs/DMMB) using in the groups with nanoparticles, DMMB (750 ng/mL) encapsulated with rhamnolipids in a 1:1 ratio, the light source consisted of a prototype built with a set of red LEDs with an energy density of 20 J/cm2. The results showed that applying PDT combined with encapsulation (RL-NPs/DMMB) was a more practical approach to inhibit Candida albicans (2 log reduction) than conventional applications, with a possible clinical application protocol.Copyright © 2024 Elsevier B.V. All rights reserved.