一些大型真菌作为传统或民间药物有着悠久的历史，为人类健康做出了重大贡献。为了发现具有潜在抗癌特性的生物活性分子，从药用大型真菌 Fomitopsis officinalis 中纯化出均质杂多糖 (FOBP90-1)。 FOBP90-1的分子量为2.87×104g/mol，主要由→6)-α-d-Galp-(1→, →2,6)-α-d-Galp-(1→ , →3)-α-l-Fucp-(1→, →6)-β-d-Glcp-(1→, α-d-Manp-(1→, 和 3-O-Me-α-l- Fucp-(1→根据UV、FT-IR、甲基化分析和NMR数据。除了其结构特性外，FOBP90-1在斑马鱼模型中显示出抗癌活性。以下机械分析发现，体内抗肿瘤作用与通过一系列涉及细胞、转基因斑马鱼、分子的实验确定，这些作用是通过 FOBP90-1 与 TLR-2、TLR-4、PD-L1 和 VEGFR-2 的相互作用介导的。所有实验结果都表明，纯化的真菌多糖 FOBP90-1 有望用作癌症治疗剂。版权所有 © 2024。由 Elsevier B.V. 出版。

Some macrofungi have a long history of being used as traditional or folk medicines, making significant contributions to human health. To discover bioactive molecules with potential anticancer properties, a homogeneous heteropolysaccharide (FOBP90-1) was purified from the medicinal macrofungus Fomitopsis officinalis. FOBP90-1 was found to have a molecular weight of 2.87 × 104 g/mol and mainly consist of →6)-α-d-Galp-(1→, →2,6)-α-d-Galp-(1→, →3)-α-l-Fucp-(1→, →6)-β-d-Glcp-(1→, α-d-Manp-(1→, and 3-O-Me-α-l-Fucp-(1 → according to UV, FT-IR, methylation analysis, and NMR data. In addition to its structural properties, FOBP90-1 displayed anticancer activity in zebrafish models. The following mechanical analysis discovered that the in vivo antitumor effect was linked to immune activation and angiogenesis inhibition. These effects were mediated by the interactions of FOBP90-1 with TLR-2, TLR-4, PD-L1, and VEGFR-2, as determined through a series of experiments involving cells, transgenic zebrafish, molecular docking simulations, and surface plasmon resonance (SPR). All the experimental findings have demonstrated that FOBP90-1, a purified fungal polysaccharide, is expected to be utilized as a cancer treatment agent.Copyright © 2024. Published by Elsevier B.V.