微泡（MV）的释放是健康和疾病中细胞间信号传导的重要现象。 MV 在癌症中的作用是多方面的，包括癌细胞存活、增殖和侵袭。在这项前瞻性研究中，我们分析了结直肠癌患者的 MV 水平，并评估了 MV 释放在早期结直肠癌和生存中的重要性。这项研究包括 98 名患者和 15 名对照者。使用单克隆抗体通过流式细胞术对人血浆中的 MV 进行表征。总 MV 和 MUC-1 阳性、组织因子 (TF) 阳性和内皮细胞来源的 MV (EMV) 的水平在统计上显着较高结肠癌患者的患病率高于对照组（p < 0.001）。此外，极早期结直肠癌患者亚组的上述 MV 水平与对照组相比也存在统计学显着差异（p < 0.01）。与低/中分化的肿瘤相比，高度分化的肿瘤具有较低水平的 MUC-1 阳性 MV (p < 0.02)、EMV (p < 0.002) 和 EMV/TF 组合 (p < 0.001)。我们的数据表明血浆中循环 MV 水平的分析可能成为结肠癌早期诊断的工具。

The release of microvesicles (MVs) is an essential phenomenon for inter-cellular signaling in health and disease. The role of MVs in cancer is multidimensional and includes cancer cell survival, proliferation, and invasion. In this prospective study, we analyzed MV levels in colorectal cancer patients and assessed the importance of MV release in early-stage colorectal cancer and survival.This study included 98 patients and 15 controls. The characterization of MVs from human plasma was performed by flow cytometry using monoclonal antibodies.The levels of total MVs and MUC-1-positive, tissue factor (TF)-positive, and endothelial cell-derived MVs (EMVs) were statistically significantly higher in the colon cancer patients than in the controls (p < 0.001). Furthermore, the subgroup of patients with very early-stage colorectal cancer also had statistically significant differences in the levels of the abovementioned MVs compared to the controls (p < 0.01). Highly differentiated tumors had lower levels of MUC-1-positive MVs (p < 0.02), EMVs (p < 0.002), and EMV/TF combinations (p < 0.001) versus those with tumors with low/intermediate differentiation.Our data demonstrate that the analysis of circulating MV levels in plasma could possibly become a tool for the early diagnosis of colon cancer at a very early stage of the disease.