对人类肝癌和乳腺癌细胞系中牛角瓜的茎、叶、果实和种子进行血管生成蛋白分析、植物化学筛选和计算机抗癌靶点验证。
Angiogenic protein profiling, phytochemical screening and in silico anti-cancer targets validation of stem, leaves, fruit, and seeds of Calotropis procera in human liver and breast cancer cell lines.
发表日期：2024 May 23
作者：
Muhammad Farooq Khan, Rawan Frhan Alanazi, Almohannad A Baabbad, Nawaf D Almoutiri, Mohammad Ahmad Wadaan
来源：
Stem Cell Research & Therapy
摘要：
抗癌药物发现的主要重点是开发对正常细胞温和且能够靶向多种抗癌途径的药物。由于某些国家的发病率和复发率最高，肝癌正在成为世界范围内的流行病。 Calotropis procera 是一种在沙特阿拉伯野生生长的旱生草本植物。由于其抗血管生成和抗癌能力，“C. procera”是开发创新抗癌药物的可行选择。然而，之前尚未进行任何研究来发现肝癌中由 C. procera 调节的血管生成和抗癌靶点。在本研究中，使用 C. procera 的叶、茎、花和种子来制备粗提物，并分馏到四种不同极性的溶剂中。这些生物活性引导的溶剂馏分有助于识别副作用最小的有用化合物。通过 GC-MS 鉴定了叶和茎中存在的植物成分。进行了计算机模拟研究，通过叶和茎提取物中存在的主要生物活性成分来预测抗癌靶点。使用人类血管生成抗体阵列来分析新的血管生成靶标。这项研究的结果表明，斑马鱼体内毒性筛选试验表明，C. procera 提取物是一种理想的抗癌药物，对正常细胞的毒性最小。本研究中确定的新型抗血管生成和抗癌靶标可用于设计抗肝癌药物。版权所有 © 2024。由 Elsevier Inc. 出版。
The main focus of anticancer drug discovery is on developing medications that are gentle on normal cells and should have the ability to target multiple anti-cancer pathways. Liver cancer is becoming a worldwide epidemic due to the highest occurring and reoccurring rate in some countries. Calotropis procera is a xerophytic herbal plant growing wildly in Saudi Arabia. Due to its anti-angiogenic and anticancer capabilities, "C. procera " is a viable option for developing innovative anticancer medicines. However, no study has been done previously, to discover angiogenic and anti-cancer targets which are regulated by C. procera in liver cancer. In this study, leaves, stems, flowers, and seeds of C. procera were used to prepare crude extracts and were fractionated into four solvents of diverse polarities. These bioactivity-guided solvent fractions helped to identify useful compounds with minimal side effects. The phytoconstituents present in the leaves and stem were identified by GC-MS. In silico studies were done to predict the anti-cancer targets by major bioactive constituents present in leaves and stem extracts. A human angiogenesis antibody array was performed to profile novel angiogenic targets. The results from this study showed that C. procera extracts are an ideal anti-cancer remedy with minimum toxicity to normal cells as revealed by zebrafish in vivo toxicity screening assays. The novel antiangiogenic and anticancer targets identified in this study could be explored to design medication against liver cancer.Copyright © 2024. Published by Elsevier Inc.