胞吐作用是设计高效纳米载体和确定细胞毒性的关键因素。然而，关于纳米颗粒的胞吐机制，特别是长链非编码RNA（lncRNA）的作用的研究尚未见报道。本研究分析了AuNPs在KYSE70细胞中的胞吐作用以及胞吐作用所涉及的分子途径。它表明纳米颗粒通过胞吐作用从细胞中经历时间依赖性释放，β-己糖胺酶的释放证实了 AuNP 通过溶酶体排出。机制研究表明，lncRNA ESCCAL-1 通过激活 MAPK 途径（包括 ERK 和 JNK 的磷酸化）在控制 AuNP 的胞吐作用中发挥着至关重要的作用。该研究表明 ESCCAL-1 介导的途径在 KYSE70 细胞中 AuNP 的胞吐作用中发挥着重要作用。这一发现对 ESCCAL-1 通过控制溶酶体介导的胞吐作用对食管癌耐药性的作用具有影响。版权所有 © 2024。由 Elsevier Inc. 出版。

Exocytosis is a critical factor for designing efficient nanocarriers and determining cytotoxicity. However, the research on the exocytosis mechanism of nanoparticles, especially the role of long non-coding RNAs (lncRNA), has not been reported. In this study, the exocytosis of AuNPs in the KYSE70 cells and the involved molecular pathways of exocytosis are analyzed. It demonstrates that nanoparticles underwent time-dependent release from the cells by exocytosis, and the release of β-hexosaminidase confirms that AuNPs are excreted through lysosomes. Mechanistic studies reveal that lncRNA ESCCAL-1 plays a vital role in controlling the exocytosis of AuNPs through activation of the MAPK pathway, including the phosphorylation of ERK and JNK. The study implies that the ESCCAL-1-mediated pathway plays an important role in the exocytosis of AuNPs in KYSE70 cells. This finding has implications for the role of ESCCAL-1 on the drug resistance of esophagus cancer by controlling lysosome-mediated exocytosis.Copyright © 2024. Published by Elsevier Inc.