近年来，纳米气泡（NB）凭借其独特的优势在肿瘤诊断和治疗领域获得了巨大的关注。然而，纳米纳米粒子由于其尺寸有限和反射截面单一，导致超声反射率低，其应用受到限制。我们通过将纳米SiO2纳米粒子封装在IR783标记的脂质中，合成了纳米级超声造影剂(IR783-SiO2NPs@NB)壳采用改进的薄膜水合方法。我们表征了其理化性质，检查了其微观形貌，评估了其稳定性和细胞毒性，并评估了其体外和体内对比增强超声成像能力。结果表明，IR783-SiO2NPs@NB 具有“甜甜圈型”复合材料微观结构，表现出均匀的粒径分布（637.2±86.4 nm），表现出优异的稳定性（30分钟），高生物相容性，显着的肿瘤特异性结合效率（99.78％），以及卓越的对比增强超声成像能力。我们新开发的多重散射具有肿瘤靶向能力的纳米粒子具有优异的对比增强成像能力，并且在实体瘤中表现出相对较长的对比增强持续时间，从而为纳米粒子的结构设计提供了一种新的方法。© 2024 Ma et al.

Recently, nanobubbles (NBs) have gained significant traction in the field of tumor diagnosis and treatment owing to their distinctive advantages. However, the application of NBs is limited due to their restricted size and singular reflection section, resulting in low ultrasonic reflection.We synthesized a nano-scale ultrasound contrast agent (IR783-SiO2NPs@NB) by encapsulating SiO2 nanoparticles in an IR783-labeled lipid shell using an improved film hydration method. We characterized its physicochemical properties, examined its microscopic morphology, evaluated its stability and cytotoxicity, and assessed its contrast-enhanced ultrasound imaging capability both in vitro and in vivo.The results show that IR783-SiO2NPs@NB had a "donut-type" composite microstructure, exhibited uniform particle size distribution (637.2 ± 86.4 nm), demonstrated excellent stability (30 min), high biocompatibility, remarkable tumor specific binding efficiency (99.78%), and an exceptional contrast-enhanced ultrasound imaging capability.Our newly developed multiple scattering NBs with tumor targeting capacity have excellent contrast-enhanced imaging capability, and they show relatively long contrast enhancement duration in solid tumors, thus providing a new approach to the structural design of NBs.© 2024 Ma et al.