锚蛋白重复结构域 53 (ANKRD53) 在维持染色体完整性和稳定性方面发挥着重要作用，而染色体不稳定与癌症相关。本研究通过整合分析，探讨ANKRD53在胃腺癌（STAD）中的潜在价值。基于在线数据库，使用RNA-seq和scRNA-seq数据进行整合分析。生物信息分析后通过RT-PCR证实ANKRD53的表达。进行 Kaplan-Meier 和 Cox 回归分析来评估 ANKRD53 在 STAD 中的预后价值。进行基因集富集分析 (GSEA) 以评估 ANKRD53 相关信号通路。此外，还研究了ANKRD53与免疫的相互作用。STAD细胞系中的RT-PCR证实，与在线数据库中的正常样本相比，STAD样本中ANKRD53表达下调。作为独立的预测生物标志物，ANKRD53 与其他临床病理学参数相结合以创建预后列线图。使用 GSEA，发现 ANKRD53 参与五个通路，包括 TGF-β 信号通路。进一步的研究表明，除了 MSI、TMB 和新抗原之外，ANKRD53 还与免疫检查点分子、免疫通路和免疫治疗相关。此外，scRNA-seq数据显示ANKRD53主要在CD8 T和树突状细胞中表达。ANKRD53是STAD的重要生物标志物，值得进一步关注。© 2024。作者。

Ankyrin repeat domain 53 (ANKRD53) plays an important role in maintaining chromosome integrity and stability, and chromosome instability is associated with cancer. Through integrative analysis, this study investigates the potential value of ANKRD53 in stomach adenocarcinoma (STAD).RNA-seq and scRNA-seq data were used for integrative analysis based on online databases. Expression of ANKRD53 was confirmed by RT-PCR after bioinformatic analysis. Kaplan-Meier and Cox regression analyses were performed to evaluate the prognostic value of ANKRD53 in STAD. Gene set enrichment analysis (GSEA) was performed to evaluate ANKRD53-related signaling pathways. In addition, the interaction of ANKRD53 with immunity was also investigated.RT-PCR in STAD cell lines confirmed that ANKRD53 was downregulated in STAD samples compared to normal samples in the online databases. As an independent predictive biomarker, ANKRD53 was combined with other clinicopathological parameters to create a prognostic nomogram. Using GSEA, ANKRD53 was found to be involved in five pathways, including the TGF-β signaling pathway. Further investigation revealed that ANKRD53 was associated with immune checkpoint molecules, immunological pathways, and immunotherapy, in addition to MSI, TMB and neoantigens. In addition, scRNA-seq data revealed that ANKRD53 is mainly expressed in CD8+ T and dendritic cells.ANKRD53 is an important biomarker for STAD that deserves further attention.© 2024. The Author(s).