谷胱甘肽过氧化物酶-4 (GPX4) 是铁死亡和脂质循环的成员。本研究旨在探讨GPX4在食管鳞癌中的表达情况及其对放射敏感性的影响。采用免疫组化染色法检测180例食管鳞癌组织及癌旁组织中GPX4的表达情况。我们分析了 GPX4 表达与 ESCC 临床参数之间的关系。采用细胞凋亡测定和集落形成测定进行体外实验，研究GPX4对ESCC细胞放射敏感性的影响。利用裸鼠异种移植模型进行体内实验，评价GPX4对食管鳞癌放射敏感性的影响。癌旁组织中GPX4的表达量低于肿瘤组织。 GPX4的表达与ESCC的病理分级显着相关。 GPX4低表达的ESCC患者的总生存时间（OS）显着长于GPX4高表达的患者。 GPX4可作为ESCC患者的独立预后因素。体内实验发现，沉默GPX4或使用GPX4抑制剂可显着抑制辐射后ESCC细胞的活力和集落形成，同时增加细胞内活性氧（ROS）水平，并显着抑制辐射后皮下异种移植物中ESCC细胞的致瘤能力.GPX4在ESCC中高表达，对于ESCC的预后评估具有潜在价值。沉默或抑制 GPX4 可以增强 ESCC 的放射敏感性。© 2024 by the Association of Clinical Scientifics, Inc.

Glutathione peroxidase-4 (GPX4) is a member of Ferroptosis and lipid circulation. This study aims to investigate the expression of GPX4 in esophageal squamous cell carcinoma and its impact on radiosensitivity.Immunohistochemistry staining was used to detect GPX4 expression in 180 samples of ESCC tissues and adjacent tissues. We analyzed the relationship between GPX4 expression and ESCC clinical parameters. In vitro experiments were conducted using apoptosis assays and colony formation assays to investigate the effect of GPX4 on the radiosensitivity of ESCC cells. In vivo experiments were carried out using a nude mouse xenograft model to evaluate the impact of GPX4 on the radiosensitivity of ESCC.GPX4 expression was lower in adjacent tissues than tumor tissues. The expression of GPX4 was significantly associated with the pathological grade of ESCC. The overall survival time (OS) of ESCC patients with low GPX4 expression was significantly longer than that of patients with high GPX4 expression. GPX4 could be used as independent prognostic factors in patients with ESCC. In vivo experiments, silencing of GPX4 or using GPX4 inhibitors significantly inhibits the viability and colony formation of ESCC cells after radiation exposure while increasing intracellular reactive oxygen species (ROS) levels, and significantly suppresses the tumorigenic ability of ESCC cells in subcutaneous xenografts after radiation exposure.GPX4 is highly expressed in ESCC, which has the potential value for prognostic assessment of ESCC. Silencing or inhibiting GPX4 can enhance the radiosensitivity of ESCC.© 2024 by the Association of Clinical Scientists, Inc.