近年来，由于死亡率上升的可怕前景，抗菌素耐药性（AMR）引起了全世界的关注。纳米材料因其在广泛的技术和生物应用中的潜力而受到研究。本研究的目的是使用曲霉属生物合成氧化锌纳米颗粒（ZnONP）。 SA17 真菌提取物，然后使用电子显微镜（TEM 和 SEM）、UV 分析、X 射线衍射 (XRD) 和傅里叶变换红外光谱 (FT-IR) 对所产生的纳米颗粒 (NP) 进行表征。 TEM 显示出平均尺寸为 7.2 nm 的球形纳米颗粒，XRD 验证了生成的 ZnONPs 的晶体性质和晶体结构特征，而 zeta 电位为 18.16 mV，表明颗粒表面带正电。 FT-IR 还用于鉴定参与 ZnONP 合成的生物分子。还研究了粗制真菌提取物及其纳米形式对几种微生物菌株和癌细胞系的抗菌和抗癌特性。对病原菌的抑菌圈直径为3～13mm，对癌细胞系的IC50值为17.65～84.55M。此外，还发现了黄酮类、酚酸类、香豆素类、有机酸类、蒽醌类、木脂素类等33种化合物。使用 UPLC-QTOF-MS/MS 对提取物进行化学分析。根据计算机模拟研究，一些分子，如 pomiferin 和 glabrol，可能可用于抗菌目的，而大豆苷元 4'-硫酸盐则显示出作为抗癌代谢物的前景。版权所有 © 2024 Abdelrahman、El Hawary、Mohsen、El Raey、Selim 、哈姆丹、加里布和哈米德。

In recent years, the world's attention has been drawn to antimicrobial resistance (AMR) because to the frightening prospect of growing death rates. Nanomaterials are being investigated due to their potential in a wide range of technical and biological applications.The purpose of this study was to biosynthesis zinc oxide nanoparticles (ZnONPs) using Aspergillus sp. SA17 fungal extract, followed by characterization of the produced nanoparticles (NP) using electron microscopy (TEM and SEM), UV-analysis, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR).The HR-TEM revealed spherical nanoparticles with an average size of 7.2 nm, and XRD validated the crystalline nature and crystal structure features of the generated ZnONPs, while the zeta potential was 18.16 mV, indicating that the particles' surfaces are positively charged. The FT-IR was also used to identify the biomolecules involved in the synthesis of ZnONPs. The antibacterial and anticancer properties of both the crude fungal extract and its nano-form against several microbial strains and cancer cell lines were also investigated. Inhibition zone diameters against pathogenic bacteria ranged from 3 to 13 mm, while IC50 values against cancer cell lines ranged from 17.65 to 84.55 M. Additionally, 33 compounds, including flavonoids, phenolic acids, coumarins, organic acids, anthraquinones, and lignans, were discovered through chemical profiling of the extract using UPLC-QTOF-MS/MS. Some molecules, such pomiferin and glabrol, may be useful for antibacterial purposes, according to in silico study, while daidzein 4'-sulfate showed promise as an anti-cancer metabolite.Copyright © 2024 Abdelrahman, El Hawary, Mohsen, El Raey, Selim, Hamdan, Ghareeb and Hamed.