口腔鳞状细胞癌（OSCC）是口腔癌最常见的亚型。在早期阶段检测口腔潜在恶性疾病 (OPMD) 对于防止其发展为 OSCC 至关重要。导致口腔鳞状细胞癌的主要因素之一是吸烟，这会导致细胞因子的产生增加。在这些细胞因子中，白细胞介素 6 (IL-6) 是一种参与炎症的免疫分子，可能作为评估 OPMD 和 OSCC 进展的有价值的指标。本研究的目的是评估两种血清中 IL6 的水平。使用酶联免疫吸附测定 (ELISA) 技术对唾液和唾液进行检测，并确定这些测量结果对口腔白斑和 OSCC 患者的预后价值。该研究涉及 45 名参与者，他们被分为三组：OSCC (15)、白斑 (15) 15），以及由健康个体组成的对照组（15）。从所有三组中的每个人收集唾液和血清样本，并使用 ELISA 方法进行分析。随后，对收集的数据进行统计分析进行评估。与白斑患者和健康对照组相比，口腔鳞癌患者唾液和血清中IL-6的水平均升高，且差异有统计学意义。 ROC（受试者工作特征）曲线分析表明，唾液IL-6是比血清IL-6更有效的检测OSCC进展的指标。随着 OSCC 和白斑病例组织学分化程度的增加，唾液 IL-6 水平也相应升高。唾液和血清 IL-6 水平都有可能作为口腔白斑和 OSCC 有价值的预后生物标志物，这表明IL-6 可能参与这些病症的发生和进展。与血清 IL-6 相比，唾液 IL-6 是一种优越的预后标志物，因为它具有非侵入性，这使其成为大规模筛查的有用工具。版权所有 © 2024，Oshin 等人。

Oral squamous cell carcinoma (OSCC) is the most prevalent subtype of oral cancer. Detecting oral potentially malignant disorders (OPMDs) in their early stages is crucial to prevent their advancement into OSCC. One of the primary factors contributing to OSCC is tobacco use, which can lead to increased production of cytokines. Among these cytokines, interleukin-6 (IL-6), an immune molecule involved in inflammation, may serve as a valuable indicator for assessing the progression of OPMDs and OSCCs.The aim of this study is to assess the levels of IL6 in both serum and saliva using the enzyme-linked immunosorbent assay (ELISA) technique and to determine the prognostic value of these measurements in individuals with oral leukoplakia and OSCC.The research involved 45 participants, who were categorized into three groups: OSCC (15), leukoplakia (15), and a control group consisting of healthy individuals (15). Saliva and serum samples were collected from each individual within all three groups and analyzed using the ELISA method. Subsequently, the gathered data underwent statistical analysis for evaluation.There were elevated levels of IL-6 in both saliva and serum among individuals with OSCC in comparison to those with leukoplakia and the healthy control group, and this difference was statistically significant. The analysis of ROC (Receiver Operating Characteristic) curves demonstrated that salivary IL-6 was a more effective indicator than serum IL-6 for detecting the advancement of OSCC. As the histological grade of differentiation increased in both OSCC and leukoplakia cases, there was a corresponding rise in salivary IL-6 levels.Both salivary and serum IL-6 levels have the potential to serve as valuable prognostic biomarkers for oral leukoplakia and OSCC which shows possible involvement of IL-6 in the development and progression of these conditions. Salivary IL-6 is a superior prognostic marker compared to serum IL-6 due to its non-invasive nature which makes it a useful tool for mass screening.Copyright © 2024, Oshin et al.