细胞毒性测定对于评估药物杀死癌细胞的功效并确定其潜在治疗价值至关重要。药物浓度的影响是细胞毒性的影响因素，其测量非常重要。本文首次提出了一种基于活细胞数量检测的终点方法，使用微波传感器进行细胞毒性测定。与荧光标记等光学方法相比，本研究使用谐振器型微波生物传感器，通过监测由于细胞密度变化而导致的电参数变化来评估药物浓度对细胞毒性的影响。最初，证实了用超纯水处理细胞以通过微波生物传感器进行细胞计数的可行性。随后，比较了 CCK-8 方法和新型微波生物传感器针对不同药物浓度产生的抑制曲线，发现它们是一致的。该协议支持基于微波的方法通过药物浓度量化细胞生长抑制的潜力。版权所有 © 2024 赵、王、石、王、江、杨、高和强。

Cytotoxicity assays are crucial for assessing the efficacy of drugs in killing cancer cells and determining their potential therapeutic value. Measurement of the effect of drug concentration, which is an influence factor on cytotoxicity, is of great importance. This paper proposes a cytotoxicity assay using microwave sensors in an end-point approach based on the detection of the number of live cells for the first time. In contrast to optical methods like fluorescent labeling, this research uses a resonator-type microwave biosensor to evaluate the effects of drug concentrations on cytotoxicity by monitoring electrical parameter changes due to varying cell densities. Initially, the feasibility of treating cells with ultrapure water for cell counting by a microwave biosensor is confirmed. Subsequently, inhibition curves generated by both the CCK-8 method and the new microwave biosensor for various drug concentrations were compared and found to be congruent. This agreement supports the potential of microwave-based methods to quantify cell growth inhibition by drug concentrations.Copyright © 2024 Zhao, Wang, Shi, Wang, Jiang, Yang, Gao and Qiang.