该研究的目的是通过评估银屑病患者唾液分泌和唾液细胞因子生物标志物来阐明银屑病对唾液腺的影响。这项研究招募了 120 名受试者，其中包括 60 名临床诊断为活动性银屑病的患者和 60 名年龄和性别与银屑病受试者相匹配的健康对照者。采用吐痰法采集所有受试者未受刺激的全唾液，检测肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）、IL-10的水平。 IL-10）通过酶联免疫吸附测定（BT实验室，上海，中国）测定。牛皮癣患者的唾液分泌量比健康对照者显着减少。与唾液分泌不足的银屑病患者唾液中促炎细胞因子（TNF-α、IFN-γ和IL-2）浓度显着升高，而抗炎细胞因子（IL-10）水平显着降低健康的受试者。我们的结果表明唾液流量与疾病严重程度呈显着负相关。在我们的研究中，测试细胞因子的唾液水平与唾液流速之间没有显着相关性。该研究的结果反映了银屑病患者唾液腺炎症导致唾液流量减少。唾液腺组织中促炎细胞因子浓度增加和抗炎细胞因子水平降低导致的炎症反应可能在影响银屑病患者唾液分泌方面发挥作用。银屑病患者未经刺激的唾液分泌随着疾病的严重程度和持续时间而减少。© 2024 International Union of Biochemistry and Molecular Biology, Inc.

The aim of the study is to expound the effect of psoriasis on salivary glands by evaluating the secretion of saliva and salivary cytokine biomarkers in patients with psoriasis. This study was conducted by recruiting 120 subjects that included 60 patients diagnosed clinically with active psoriasis and 60 healthy controls who were age and gender matched to psoriatic subjects. Unstimulated whole saliva was collected from all the subjects by spitting method, and levels of tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin-2 (IL-2), and IL-10 (IL-10) were determined via enzyme-linked immunosorbent assay (BT Lab, Shanghai, China). Secretion of saliva in psoriasis patients was considerably reduced than in healthy controls. The concentrations of pro-inflammatory cytokines (TNF-α, IFN-γ, and IL-2) were significantly increased, whereas level of anti-inflammatory cytokine (IL-10) was markedly decreased in the saliva of psoriasis patients with hyposalivation compared to healthy subjects. Our results demonstrated significant negative correlation of salivary flow rates with the disease severity. No significant correlations were obtained between salivary levels of tested cytokines and salivary flow rates in our study. Findings of the study reflect inflammation of salivary glands with reduced salivary flow rates in psoriasis patients. The inflammatory responses in salivary gland tissues by virtue of increased pro-inflammatory cytokines concentrations together with lower anti-inflammatory cytokine levels may have a role in affecting the saliva secretion in psoriasis patients. Secretion of unstimulated saliva in psoriasis patients decreases with the severity and duration of the disease.© 2024 International Union of Biochemistry and Molecular Biology, Inc.