乳腺肿瘤是完整雌性犬中最常见的肿瘤类型。人们高度寻求以肿瘤细胞为目标而不影响正常细胞的新疗法。炭疽杆菌毒素经过重新设计，可靶向表达尿激酶纤溶酶原激活剂和金属蛋白酶的肿瘤细胞。在本实验室前期的研究中，重组炭疽毒素对犬口腔粘膜黑色素瘤和犬骨肉瘤细胞具有抑制作用。在这项研究中，用不同浓度的重组炭疽毒素成分处理五种犬肿瘤上皮细胞系（四种腺癌和一种腺瘤）和一种非肿瘤性犬乳腺上皮细胞系。使用 MTT 测定和半最大抑制浓度 (IC50) 值对细胞活力进行定量。当 IC50 低于 5000 ng/ml 时，细胞系被认为是敏感的。一种犬乳腺癌细胞系和一种乳腺腺瘤细胞系在治疗后表现出活力显着下降，而非肿瘤细胞系则具有耐药性。我们的结论是，重新设计的炭疽毒素可被视为犬乳腺肿瘤的靶向治疗，同时保留正常的犬乳腺上皮细胞。© 2024。作者获得 Springer Nature B.V. 的独家许可。

Mammary tumors are the most frequent type of neoplasms in intact female dogs. New therapies that target neoplastic cells without affecting normal cells are highly sought. The Bacillus anthracis toxin has been reengineered to target tumor cells that express urokinase plasminogen activators and metalloproteinases. In previous studies carried out in our laboratory, the reengineered anthrax toxin had inhibitory effects on canine oral mucosal melanoma and canine osteosarcoma cells. In this study, five canine neoplastic epithelial cell lines (four adenocarcinomas and one adenoma) and one non-neoplastic canine mammary epithelial cell line were treated with different concentrations of reengineered anthrax toxin components. Cell viability was quantified using an MTT assay and half-maximal inhibitory concentration (IC50) values. Cell lines were considered sensitive when the IC50 was lower than 5000 ng/ml. One canine mammary adenocarcinoma cell line and one mammary adenoma cell line showed significantly decreased viability after treatment, whereas the non-neoplastic cell line was resistant. We conclude that the reengineered anthrax toxin may be considered a targeted therapy for canine mammary neoplasms while preserving normal canine mammary epithelial cells.© 2024. The Author(s), under exclusive licence to Springer Nature B.V.