黑色素瘤作为一种高度侵袭性的皮肤癌，其治疗失败且预后不良，并且对传统疗法过度耐药，这促使人们迫切寻找更有效的治疗工具。因此，为了提高治疗效率并减少传统给药方式的副作用，将光动力疗法作为一种有前途的治疗方法与新型胶体透皮纳米平台的选择性和生物相容性相结合以有效递送混合货物变得至关重要对黑色素瘤细胞具有协同作用。指定了与 L-α-磷脂酰胆碱、胆酸钠、Pluronic® P123 和胆固醇共同稳定的自组装胆囊体，并使用动态和电泳光散射研究了胶体囊泡的稳定性，也以细胞培养基（Dulbecco's Modified Eagle's Medium）的形式提供。经 UV-Vis、ATR-FTIR 和荧光光谱证实，混合化合物 - 经典光敏剂（亚甲基蓝）和互补的天然多酚剂（姜黄素）已成功共负载。使用正常角质形成细胞和黑色素瘤癌细胞进行体外细胞毒性和光毒性实验，证明了装载双货物的聚合物功能化胆囊体的生物相容性和有用性。对人皮肤上皮 (A375) 和恶性 (Me45) 黑色素瘤的体外生物成像和免疫荧光研究细胞系证实了聚乙二醇化胆汁体表面的保护作用。这种效应在细胞毒性实验中得到证实，也在人皮肤 (HaCaT) 角质形成细胞上进行了测定。流式细胞术实验表明，与未负载的 MB 和 CUR 分子相比，封装的混合货物的吸收增强，以及所获得的纳米载体对肿瘤细胞系的选择性。照射后 24 小时提供的植物光动力作用显示，与 A375 细胞系相比，纳米平台对 Me45 细胞的影响更显着，导致细胞存活率低于对照的 20%。因此，我们建立了一个创新和通过植物光动力疗法和新型双糖体来源纳米光敏剂的协同作用，治疗潜在的转移性黑色素瘤的有效策略。© 2024 Waglewska 等人。

Numerous failures in melanoma treatment as a highly aggressive form of skin cancer with an unfavorable prognosis and excessive resistance to conventional therapies are prompting an urgent search for more effective therapeutic tools. Consequently, to increase the treatment efficiency and to reduce the side effects of traditional administration ways, herein, it has become crucial to combine photodynamic therapy as a promising therapeutic approach with the selectivity and biocompatibility of a novel colloidal transdermal nanoplatform for effective delivery of hybrid cargo with synergistic effects on melanoma cells.The self-assembled bilosomes, co-stabilized with L-α-phosphatidylcholine, sodium cholate, Pluronic® P123, and cholesterol, were designated, and the stability of colloidal vesicles was studied using dynamic and electrophoretic light scattering, also provided in cell culture medium (Dulbecco's Modified Eagle's Medium). The hybrid compounds - a classical photosensitizer (Methylene Blue) along with a complementary natural polyphenolic agent (curcumin), were successfully co-loaded, as confirmed by UV-Vis, ATR-FTIR, and fluorescent spectroscopies. The biocompatibility and usefulness of the polymer functionalized bilosome with loaded double cargo were demonstrated in vitro cyto- and phototoxicity experiments using normal keratinocytes and melanoma cancer cells.The in vitro bioimaging and immunofluorescence study upon human skin epithelial (A375) and malignant (Me45) melanoma cell lines established the protective effect of the PEGylated bilosome surface. This effect was confirmed in cytotoxicity experiments, also determined on human cutaneous (HaCaT) keratinocytes. The flow cytometry experiments indicated the enhanced uptake of the encapsulated hybrid cargo compared to the non-loaded MB and CUR molecules, as well as a selectivity of the obtained nanocarriers upon tumor cell lines. The phyto-photodynamic action provided 24h-post irradiation revealed a more significant influence of the nanoplatform on Me45 cells in contrast to the A375 cell line, causing the cell viability rate below 20% of the control.As a result, we established an innovative and effective strategy for potential metastatic melanoma treatment through the synergism of phyto-photodynamic therapy and novel bilosomal-origin nanophotosensitizers.© 2024 Waglewska et al.