在全球范围内，抗菌药物耐药性是公共卫生的主要危害。曾经很容易用抗生素治疗的感染变得越来越难以控制，导致长期患病、死亡率增加和医疗费用更高。本研究旨在评估抗菌药物，特别是抗甲氧西林金黄色葡萄球菌 (MRSA) 和抗癌药物AHA 不同提取物的特性。 AHA 对两种革兰氏阴性菌株（大肠杆菌和肺炎克雷伯菌）、两种革兰氏阳性菌株（耐甲氧西林金黄色葡萄球菌（ MRSA）和金黄色葡萄球菌）。使用良好扩散技术进行抗菌活性的初步筛选。随后，通过肉汤稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。采用MTT法对人结直肠癌细胞系（HCT-116）进行体外抗癌试验。在所有提取物中，AHA正己烷提取物对金黄色葡萄球菌最有效，抑菌圈最高（24.67 mm） ± 0.58）与标准抗生素（红霉素，24.00 mm）相比，然后是针对 MRSA 的甲醇提取物（24.00 mm ± 1.73）。 AHA 的甲醇提取物对 MRSA 显示出最高的抗菌活性。 AHA 甲醇提取物对 MRSA 的 MIC 和 MBC 结果分别为 1.17 ± 1.09 和 9.375 ± 0.0 mg/ml，证明具有治疗显着的抗菌活性。乙酸乙酯提取物对大肠杆菌和肺炎克雷伯菌没有抗菌活性。结果表明，AHA 的甲醇提取物对结直肠癌细胞系 (HCT-116) 表现出最高的功效，IC50 值为 126.61 ± 13.35 μg/ml。这些结果表明，AHA 的甲醇提取物可被视为一种潜在的抗菌和抗癌化合物来源。

Globally, resistance to antimicrobial drugs is a major hazard to public health. Infections that were once easily treatable with antibiotics are becoming harder to control, leading to prolonged illnesses, increased mortality rates, and higher healthcare costs.This study intended to assess the antimicrobial, specifically the anti-Methicillin resistant Staphylococcus aureus (MRSA), and anticancer properties of different extracts obtained from A. herba-alba (AHA).The antibacterial tests of AHA were performed on two Gram-negative bacterial strains (Escherichia coli and Klebsiella pneumonia), two Gram-positive bacterial strains (Methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus). Initial screening for antibacterial activities was conducted using the well diffusion technique. Subsequently, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined through the broth-dilution assay. The anticancer test was carried out in vitro on a human colorectal carcinoma cell line (HCT-116) using MTT assay.Among all extracts, n-hexane extract of AHA was the most effective against S. aureus with the highest inhibition zone (24.67 mm ± 0.58) compared to standard antibiotic (erythromycin, 24.00 mm) followed by the methanolic extract against MRSA (24.00 mm ± 1.73). The methanol extract of AHA showed the highest antibacterial activity against MRSA. The results of MIC and MBC of the AHA methanol extract against MRSA were 1.17 ± 1.09 and 9.375 ± 0.0 mg/ml, respectively, demonstrating therapeutically significant antibacterial activity. Ethyl acetate extract has no antibacterial activity against E. coli and K. pneumonia. The findings indicated that the methanol extract of AHA exhibited the highest efficacy against the colorectal carcinoma cell line (HCT-116), with an IC50 value of 126.61 ± 13.35 μg/ml.These findings suggest that the methanol extract of AHA could be considered as a potential agent to serve as a source of antibacterial and anticancer compounds.