本研究旨在探讨Aaptos suberitoides的乙酸乙酯提取物对结直肠癌细胞（DLD-1）和小鼠成纤维细胞（NIH-3T3）的细胞毒性作用。 suberitoides 采自印度尼西亚北苏拉威西布纳肯国家公园普图斯岛，经浸渍和乙酸乙酯提取处理。基于薄层色谱 (TLC) 对海绵提取物进行表征，然后使用 LCMS/MS 分析进行鉴定。用乙酸乙酯提取物处理DLD-1和NIH-3T3细胞，然后进行3-[4,5-二甲基噻唑-2-基]-2.5二苯基溴化四唑（MTT）测定以评估其细胞毒性作用。LCMS/MS分析表明，该提取物中最丰富的化合物被鉴定为肉毒碱 (1)。此外，本研究还表明，A. suberitoides 的活性乙酸乙酯部分对结直肠癌 DLD-1 细胞具有细胞毒作用，IC50 值为 9.597 µg/mL，高于 NIH-3T3 细胞，IC50 值为 12.23 µg/mL因此，A. suberitoides 的活性乙酸乙酯部分被认为对癌细胞比正常细胞更具毒性。这项研究提供了第一个证据，支持 A. suberitoides 海绵提取物的乙酸乙酯提取物可开发为结直肠抗癌药物代理人。

This study aimed to investigate the cytotoxicity effect of the ethyl acetate extract of Aaptos suberitoides on colorectal cancer cells (DLD-1) and murine fibroblast cells (NIH-3T3).A. suberitoides was collected from Putus Island, Bunaken National Park, North Sulawesi, Indonesia, and was processed with maceration and ethyl acetate extraction. The sponge extract was characterized based on Thin Layer Chromatography (TLC) and then identified by using LCMS/MS analysis. DLD-1 and NIH-3T3 cells were treated with the ethyl acetate extract and then followed by 3- [4, 5-dimethylthiazol-2-yl] -2.5 diphenyl tetrazolium bromide (MTT) assay to assess their cytotoxicity effect.LCMS/MS analysis showed that the most abundant compounds in this extract were identified as aaptamine (1). Furthermore, this study revealed that the active ethyl acetate fraction of A. suberitoides has cytotoxic effects in colorectal cancer DLD-1 cells with an IC50 value of 9.597 µg/mL, higher than NIH-3T3 cells with an IC50 value of 12.23 µg/mL Thus, the active ethyl acetate fraction of A. suberitoides is considered more toxic to cancer cells than normal cells.This study provides the first evidence to support the role of the ethyl acetate extract of A. suberitoides sponge extracts to be developed as a colorectal anticancer agent.