多形性胶质母细胞瘤（GBM）因其高侵袭性和不良预后而构成重大挑战，现有治疗方法在延长生存率方面效果有限。本研究旨在评估 Aaptos suberitoides 提取物和组分对 U87 细胞系（作为 GBM 的代表性模型）的抗癌特性。U87 细胞用 Aaptos suberitoides 的乙醇提取物处理，特别是两种提取物（OAA-1 和 OAA- 2) 和从 Pramuka 岛和 Tinjil 岛采集的标本中分离出的一种乙酸乙酯组分 (EA)。评估包括显微镜观察和MTT测定以确定IC50。随后，通过细胞凋亡和细胞周期测定研究了抗增殖作用。提取物对 U87 细胞具有细胞毒活性，OAA-1 和 OAA-2 的 IC50 值分别为 35.78 μg/mL 和 25.38 μg/mL。 OAA-1 在 50 μg/mL 时显着诱导细胞凋亡并诱导细胞周期停滞。另一方面，OAA-2 虽然也显着诱导细胞凋亡，但对细胞周期停滞的影响较小。相比之下，EA 在 100 μg/mL 浓度下诱导显着的细胞凋亡。Aaptos suberitoides 的乙醇提取物和乙酸乙酯部分成为多形性胶质母细胞瘤癌症治疗的有希望的候选者，显示出抑制细胞增殖和诱导细胞凋亡的潜力。

Glioblastoma Multiforme (GBM) poses a significant challenge due to its high aggressiveness and unfavorable prognosis, with existing treatments demonstrating limited efficacy in prolonging survival rates. This study aimed to assess the anticancer properties of Aaptos suberitoides extracts and fraction on the U87 cell line, serving as a representative model for GBM.U87 cells were treated with ethanol extracts derived from Aaptos suberitoides, specifically two extracts (OAA-1 and OAA-2) and one ethyl acetate fraction (EA) isolated from specimens collected on Pramuka Island and Tinjil Island. The evaluation encompased microscopic observation and MTT assay to determine the IC50. Subsequently, antiproliferative effects were investigated through apoptosis and cell cycle assays.The extract demonstrated cytotoxic activity against U87 cells, with OAA-1 and OAA-2 exhibiting IC50 values of 35.78 μg/mL and 25.38 μg/mL, respectively. OAA-1 notably induced apoptosis at 50 μg/mL and induced cell cycle arrest. On other hand, OAA-2, while also inducing apoptosis significantly, had a lesser impact on cell cycle arrest. In contrast, EA induced significant apoptosis at a concentration of 100 μg/mL.The ethanol extracts and the ethyl acetate fraction of Aaptos suberitoides emerged as a promising candidate for Glioblastoma Multiforme cancer therapy, showing potential in inhibiting cell proliferation and inducing apoptosis.