大脑类器官在模拟人类大脑发育和发病机制方面具有巨大潜力。它们概括了发育中大脑的转录轨迹、细胞多样性、组织结构和功能的某些方面。在这篇综述中，我们探索了控制分子、细胞和组织水平输入以实现高保真脑类器官的工程策略。我们回顾了脑类器官在神经疾病建模和新兴生物工程方法中的应用，以改进多尺度的数据收集和特征提取。多尺度工程策略和分析方法的整合在推进对神经系统疾病的深入了解和加速药物开发方面具有巨大潜力。版权所有 © 2024。由 Elsevier B.V. 出版。

Brain organoids hold great potential for modeling human brain development and pathogenesis. They recapitulate certain aspects of the transcriptional trajectory, cellular diversity, tissue architecture and functions of the developing brain. In this review, we explore the engineering strategies to control the molecular-, cellular- and tissue-level inputs to achieve high-fidelity brain organoids. We review the application of brain organoids in neural disorder modeling and emerging bioengineering methods to improve data collection and feature extraction at multiscale. The integration of multiscale engineering strategies and analytical methods has significant potential to advance insight into neurological disorders and accelerate drug development.Copyright © 2024. Published by Elsevier B.V.