肺腺癌（LUAD）是最常见、最具侵袭性的癌症，发病率很高。 N1特异性假尿苷甲基转移酶（EMG1）是一种高度保守的核仁蛋白，在核糖体的生物发育中发挥着重要作用。然而，EMG1 在 LUAD 进展中的作用仍不清楚。使用实时聚合酶链反应 (PCR) 和蛋白质印迹法对 ​​LUAD 细胞、LUAD 组织和邻近非癌组织中 EMG1 的表达进行定量。在体外和体内探讨了 EMG1 在 LUAD 细胞增殖、迁移、侵袭和致瘤性中的作用。蛋白质印迹分析 EMG1 调节 LUAD 生物学功能的潜在分子机制。使用GEPIA、UALCAN、cBioPortal、LinkedOmics和Kaplan-Meier Plotter等一系列数据库分析EMG1表达及其对肿瘤预后的影响。与正常组织相比，LUAD患者中EMG1表达升高，且EMG1表达与预后强相关LUAD 患者。 EMG1表达与年龄、性别、N分期、T分期和病理分期相关。 EMG1表达与MRPL51、PHB2、SNRPG、ATP5MD和TPI1呈强正相关，与MACF1、DOCK9、RAPGEF2、SYNJ1和KIDINS220呈强负相关，EMG1及相关基因的主要富集途径包括细胞周期、DNA复制和癌症信号通路中的通路。 LUAD 细胞系和组织中 EMG1 表达水平显着增加。 EMG1 的敲低可以抑制 LUAD 细胞的增殖、迁移、侵袭和致瘤性。此外，EMG1过表达可以促进LUAD细胞的增殖、迁移和侵袭。 EMG1的高表达预示着LUAD患者预后不良，并且EMG1可能通过参与LUAD免疫细胞的浸润而在肿瘤微环境中发挥致癌作用。EMG1通过直接介导Akt/mTOR/p70s6k信号通路的激活来调节LUAD中的多种功能。结果表明 EMG1 可能是评估 LUAD 预后和免疫细胞浸润的新型生物标志物。版权所有 © 2024 作者。由 Elsevier B.V. 出版。保留所有权利。

Lung adenocarcinoma (LUAD) is the most common and aggressive cancer with a high incidence. N1-specific pseudouridine methyltransferase (EMG1), a highly conserved nucleolus protein, plays an important role in the biological development of ribosomes. However, the role of EMG1 in the progression of LUAD is still unclear.The expression of EMG1 in LUAD cells, and LUAD tissues, and adjacent noncancerous tissues was quantified using real-time polymerase chain reaction (PCR) and western blotting. The roles of EMG1 in LUAD cell proliferation, migration, invasion and tumorigenicity were explored in vitro and in vivo. Western blot analysis to underlying molecular mechanism of EMG1 regulating the biological function of LUAD. EMG1 expression and its impact on tumor prognosis were analyzed using a range of databases including GEPIA, UALCAN, cBioPortal, LinkedOmics, and Kaplan-Meier Plotter.EMG1 expression was elevated in LUAD patients compared to normal tissues, and EMG1 expression was strongly correlated with prognosis in LUAD patients. EMG1 expression correlated with age, gender, N stage, T stage, and pathologic stage. EMG1 expression was strongly positively correlated with MRPL51, PHB2, SNRPG, ATP5MD, and TPI1, and strongly negatively correlated with MACF1, DOCK9, RAPGEF2, SYNJ1, and KIDINS220, the major enrichment pathways for EMG1 and related genes include Cell cycle, DNA Replication and Pathways in cancer signaling pathways. EMG1 expression level was significantly increased in LUAD cell lines and tissues. Knockdown of EMG1 could inhibit LUAD cell proliferation, migration, invasion, and tumorigenicity. Besides, EMG1 overexpression could promote LUAD cell proliferation, migration, and invasion. High expression of EMG1 predicts poor prognosis in LUAD patients, and EMG1 may play an oncogenic role in the tumor microenvironment by participating in the infiltration of LUAD immune cells.EMG1 regulated various functions in LUAD by directly mediating Akt/mTOR/p70s6k signaling pathways activation. The results suggest that EMG1 may be a novel biomarker for assessing prognosis and immune cell infiltration in LUAD.Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.