磷酸三苯酯 (TPP) 暴露促进胃癌细胞的增殖和迁移能力:基因表达和通路分析的见解。
Triphenyl phosphate (TPP) exposure promotes proliferation and migration capabilities of gastric cancer cells: Insights from gene expression and pathway analysis.
发表日期:2024 Jun 28
作者:
Zhongshi Hong, Yi Wang, Xiaojing Chen, Yuze Wu, Yi Qiu, Xian Deng, Mingliang Chen, Chunxiao Wang, Yachen Li, Chengzhi Qiu
来源:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
摘要:
胃癌是癌症相关死亡的主要原因,受遗传和环境因素的影响。磷酸三苯酯 (TPP) 是一种常见的阻燃剂,但其对健康的影响仍有待彻底了解。通过检查基因表达模式并开发预测模型来探索 TPP 暴露与胃癌之间的联系。基因表达数据来源于 The Cancer基因组图谱 (TCGA) 和比较毒理学基因组数据库 (CTD)。采用基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径进行分析。使用单样本基因集富集分析 (ssGSEA) 来获得磷酸盐阻燃剂相关分数。通过差异分析、单变量 COX 回归和 LASSO 回归构建了预测模型。进行分子对接以评估蛋白质与 TPP 的相互作用。ssGSEA 确定了与胃癌中磷酸盐阻燃剂相关的评分,该评分与免疫相关特征有很强的相关性。与 TPP 相关的几个基因已被鉴定并用于开发具有临床意义的预后模型。分子对接显示TPP与脂质代谢相关基因MTTP具有高结合亲和力。通路分析表明,TPP 暴露通过脂质代谢过程导致胃癌。该研究建立了 TPP 暴露与胃癌发病之间的潜在相关性,并查明了所涉及的关键基因和通路。这强调了环境因素在胃癌研究中的重要性,并为临床应用提供了潜在的诊断工具。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。
Gastric cancer is a leading cause of cancer-related deaths influenced by both genetic and environmental factors. Triphenyl phosphate (TPP) is a prevalent flame retardant, but its health implications remain to be thoroughly understood.To explore the link between TPP exposure and gastric cancer by examining gene expression patterns and developing a predictive model.Gene expression data were sourced from The Cancer Genome Atlas (TCGA) and the Comparative Toxicogenomics Database (CTD). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were employed for analysis. Single-sample Gene Set Enrichment Analysis (ssGSEA) was used to obtain phosphate flame retardant-related scores. A predictive model was constructed through differential analysis, univariate COX regression, and LASSO regression. Molecular docking was performed to assess protein interactions with TPP.ssGSEA identified scores related to phosphate flame retardants in gastric cancer, which had a strong association with immune-related traits. Several genes associated with TPP were identified and used to develop a prognostic model that has clinical significance. Molecular docking showed a high binding affinity of TPP with MTTP, a gene related to lipid metabolism. Pathway analysis indicated that TPP exposure contributes to gastric cancer through lipid metabolic processes.The study establishes a potential correlation between TPP exposure and gastric cancer onset, pinpointing key genes and pathways involved. This underscores the significance of environmental factors in gastric cancer research and presents a potential diagnostic tool for clinical application.Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.