增强抗癌药物向癌细胞的靶向递送的最新挑战是提高药物递送系统的生物利用度和治疗功效，同时最大限度地减少其全身副作用。在本研究中，在羟基磷灰石纳米粒子（HAP）存在下，采用原位方法合成了MIL-88（Fe）金属有机骨架，制备了HAP/MIL-88（Fe）（HM）纳米复合材料。然后通过 Steglich 酯化方法用甘露糖 (M) 作为抗癌受体对其进行功能化。各种分析证实了 MHM 的成功合成。为了进行药物释放研究，将 5-Fu 加载到 MHM 中，然后在 MHM 上涂上透明质酸 (HA) 水凝胶膜。表征分析验证了所得 HA/5-Fu-MHM 水凝胶膜的结构。体外药物释放实验表明，HA/5-Fu-MHM中5-Fu药物的释放可以通过pH来控制，降低其在胃酸性环境中的释放速率，而在肠道环境中增加其释放速率。 HA/5-Fu-MHM水凝胶膜对HT29癌细胞的细胞毒性结果显示，由于其结构中的甘露糖和透明质酸，细胞毒性增强，从而触发了双靶向药物递送系统。获得的结果表明所制备的水凝胶薄膜可以成为结肠癌治疗的有前途的生物平台。版权所有©2024。由Elsevier B.V.出版。

The recent challenge in enhancing the targeted delivery of anticancer drugs to cancer cells is improving the bioavailability and therapeutic efficacy of drug delivery systems while minimizing their systemic side effects. In this study, the MIL-88(Fe) metal-organic framework was synthesized using the in situ method in the presence of hydroxyapatite nanoparticles (HAP) toward the HAP/MIL-88(Fe) (HM) nanocomposite preparation. It was then functionalized with mannose (M) as an anticancer receptor through the Steglich esterification method. Various analyses confirmed the successful synthesis of MHM. For drug release investigation, 5-Fu was loaded into the MHM, which was then coated with a hyaluronic acid (HA) hydrogel film. Characterization analyses verified the structure of the resulting HA/5-Fu-MHM hydrogel film. In vitro drug release experiments showed that the release of 5-Fu drug from HA/5-Fu-MHM could be controlled with pH, reducing its release rate in the acidic environment of the stomach while increasing it in the intestinal environment. Cytotoxicity results of the HA/5-Fu-MHM hydrogel film against HT29 cancer cells showed enhanced cytotoxicity due to the mannose and hyaluronic acid in its structure, which triggers a dual-targeted drug delivery system. The obtained results indicate that the prepared hydrogel films can be a promising bio-platform for colon cancer treatment.Copyright © 2024. Published by Elsevier B.V.