通过壳聚糖与2-(4-甲酰基苯氧基)-N-苯基乙酰胺和N-(4-溴苯基)-2-(4-甲酰基苯氧基)乙酰胺缩合合成了两种壳聚糖席夫碱，分别表示为Cs-SBA和Cs-SBBr 。使用 FTIR 和 1HNMR 对所得壳聚糖衍生物的分子结构进行了表征，并通过 TGA 研究了它们的热性能。这些衍生物用三聚磷酸钠（TPP）处理以产生铯希夫碱纳米颗粒。通过 FTIR、XRD、TEM 和 zeta 电位分析测定纳米颗粒的物理化学性质。评估了抗幽门螺杆菌（H. pylori）的抗菌作用，结果表明抗 H. Cs-SBA 和 Cs-SBBr 纳米粒子（Cs-SBA NPs 和 Cs-SBBr NPs）的幽门螺杆菌活性最小抑制浓度 MIC 值分别为 15.62±0.05 和 3.9±0.03μg/mL。测试了生物活性更好的纳米颗粒 Cs-SBBr NP 的环氧合酶（COX-1 和 COX-2）抑制潜力。 Cs-SBBr NPs 对 COX-2 的 COX 酶抑制活性 (IC50 4.5±0.165μg/mL) 高于传统吲哚美辛 (IC50 0.08±0.003μg/mL) 和塞来昔布 (IC50 0.79±0.029μg/mL)。此外，Cs-SBBr NPs的细胞毒性测试显示对Vero细胞(CCL-81)具有细胞毒性作用，IC50=17.95±0.12μg/mL，被认为是安全的化合物。因此，Cs-SBBr NPs可能成为治疗幽门螺杆菌和预防胃癌的替代方案。版权所有©2024。由Elsevier B.V.出版。

Two chitosan Schiff bases were synthesized by condensation of chitosan with 2-(4-formylphenoxy)-N-phenylacetamide and N-(4-bromophenyl)-2-(4-formylphenoxy) acetamide denoted as Cs-SBA and Cs-SBBr, respectively. The molecular structures of the resulting chitosan derivatives were characterized using FTIR and 1HNMR and their thermal properties were investigated by TGA. These derivatives were treated with sodium tripolyphosphate (TPP) to produce Cs Schiff base nanoparticles. The nanoparticles physicochemical properties were determined by FTIR, XRD, TEM, and zeta potential analysis. The antimicrobial action against Helicobacter pylori (H. pylori) was evaluated and the results indicated that the anti-H. pylori activity had minimal inhibitory concentration MIC values of 15.62 ± 0.05 and 3.9 ± 0.03 μg/mL for Cs-SBA and Cs-SBBr nanoparticles (Cs-SBA NPs and Cs-SBBr NPs), respectively. The better biologically active nanoparticles, Cs-SBBr NPs, were tested for their cyclooxygenases (COX-1 and COX-2) inhibitory potential. Cs-SBBr NPs demonstrated COX enzyme inhibition activity against COX-2 (IC50 4.5 ± 0.165 μg/mL) higher than the conventional Indomethacin (IC50 0.08 ± 0.003 μg/mL), and Celecoxib (IC50 0.79 ± 0.029 μg/mL). Additionally, the cytotoxicity test of Cs-SBBr NPs showed cytotoxic effect on Vero cells (CCL-81) with IC50 = 17.95 ± 0.12 μg/mL which is regarded as a safe compound. Therefore, Cs-SBBr NPs may become an alternative to cure H. pylori and prevent gastric cancer.Copyright © 2024. Published by Elsevier B.V.